Pseudomonas asiatica sp. nov., isolated from hospitalized patients in Japan and Myanmar
Pseudomonas asiatica sp. nov., isolated from hospitalized patients in Japan and Myanmar
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DOI：
10.1099/ijsem.0.003316
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发表时间：
2019-05-01
影响因子：
2.8
通讯作者：
Kirikae, Teruo
中科院分区：
文献类型：
--
作者：
Tohya, Mari;Watanabe, Shin;Kirikae, Teruo
从日本冲绳某医院住院患者粪便标本中分离到一株革兰氏阴性、需氧、杆状、不产芽孢的新菌株RYU 5（T）。RYU 5（T）的最适生长温度为30 ℃。基于管家基因（包括16 S rRNA、rpoB、rpoD和gyrB基因）序列的系统发育分析表明，RYU 5（T）属于恶臭假单胞菌群，与蒙氏假单胞菌和恶臭假单胞菌的亲缘关系较近。全基因组比较，使用平均核苷酸同一性和数字DNA-DNA杂交，证实菌株RYU 5（T）应被归类为假单胞菌的一个新物种。表型鉴定试验表明，利用D-甘露糖、D-丝氨酸、L-阿拉伯糖和D-果糖可以将该菌株与假单胞菌属的其他相关种区分开来。基于遗传和表型证据，菌株RYU 5 T应被分类为一个新种，并建议将其命名为亚洲假单胞菌Pseudomonasiatica sp. nov.。模式菌株为RYU 5（T）（=DSM 107182（T），=JCM 32716（T）），DNA G+C含量为62.25mol%。
A novel Gram-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, RYU5(T), was isolated from a stool sample of an inpatient at a hospital in Okinawa, Japan. The optimal growth temperature of RYU5(T) was 30 degrees C. Phylogenetic analysis based on the sequences of housekeeping genes, including the 16S rRNA, rpoB, rpoD and gyrB genes, showed that RYU5(T) was a member of the Pseudomonas putida group and was located close to Pseudomonas monteilii and P. putida. Whole-genome comparisons, using average nucleotide identity and digital DNA-DNA hybridization, confirmed that strain RYU5(T) should be classified as a novel species of Pseudomonas. Phenotypic characterization tests showed that utilization of D-mannose, D-serine, L-arabinose and D-fructose could distinguish this strain from other related species of the genus Pseudomonas. Based on genetic and phenotypic evidence, strain RYU5 T should be classified as a novel species, for which the name Pseudomonas asiatica sp. nov. is proposed. The type strain is RYU5(T) (=DSM 107182(T), =JCM 32716(T)), with a DNA G+C content of 62.25 mol%.