THE ATP-SENSITIVITY OF K+ CHANNELS IN RAT PANCREATIC B-CELLS IS MODULATED BY ADP
THE ATP-SENSITIVITY OF K+ CHANNELS IN RAT PANCREATIC B-CELLS IS MODULATED BY ADP
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DOI：
10.1016/0014-5793(86)81533-2
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发表时间：
1986-11-10
期刊：
影响因子：
3.5
通讯作者：
ASHCROFT, FM
中科院分区：
文献类型：
--
作者：
KAKEI, M;KELLY, RP;ASHCROFT, FM
用膜片钳技术研究了大鼠胰腺B细胞由内而外膜膜片上ATP敏感性K+通道的变化。ATP诱导的通道抑制的剂量-反应曲线在ADP（2 mM）的存在下移动到更高的浓度。在无葡萄糖溶液中，ATP的总细胞内浓度为3.8 mM，ADP为1.5 mM;葡萄糖（20 mM）增加ATP，减少ADP约1.5 mM。百分之四十这些结果表明，ADP和ATP都可能参与调节完整B细胞葡萄糖敏感性K+通道的活性。
ATP-sensitive K+channels in inside-out membrane patches from dispersed rat pancreatic B-cells were studied using patch-clamp methods. The dose-response curve for ATP-induced channel inhibition was shifted to higher concentrations in the presence of ADP (2 mM). In glucose-free solution, the total intracellular concentration of ATP was 3.8 mM and of ADP 1.5 mM; glucose (20 mM) increased ATP and decreased ADP by approx. 40%. These results suggest that both ADP and ATP may be involved in regulating the activity of the glucose-sensitive K+channel in intact B-cells.