CARRIER STATE OF BACILLUS SUBTILIS INFECTED WITH TRANSDUCING BACTERIOPHAGE SP10
CARRIER STATE OF BACILLUS SUBTILIS INFECTED WITH TRANSDUCING BACTERIOPHAGE SP10
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DOI：
10.1016/0042-6822(65)90200-x
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发表时间：
1965-01-01
期刊：
影响因子：
3.7
通讯作者：
STRAUSS, B
中科院分区：
文献类型：
--
作者：
BOTT, K;STRAUSS, B
噬菌体SP10可感染枯草芽孢杆菌形成载体系统。受感染的细菌经过六次或更多次连续的单菌落分离后产生受感染的生物体。从这种受感染的培养物中产生的约60%的孢子产生产生噬菌体的克隆，即使用抗血清处理并在80[度]加热后，这些孢子仍保持其产生噬菌体的能力。载体孢子在营养液中培养4小时，每个细菌有10多个感染中心。在含有SP10抗血清的培养基中培养受感染的生物体会导致噬菌体的丧失和对噬菌体感染的免疫力丧失。在被感染的细菌中可以检测到具有病毒DNA密度特征的DNA。感染细菌的提取物含有噬菌体诱导的脱氧核糖核酸酶活性，在体外攻击噬菌体和细菌DNA。因此，噬菌体-细菌复合体最好被描述为载体状态。噬菌体和枯草芽孢杆菌的DNA在化学上是不同的。酶法制备的互补病毒RNA与细菌DNA无同源性。这种转导噬菌体DNA与其宿主DNA缺乏同源性，并且未能形成真正的溶原关系，这加强了先前的结论，即转导细菌DNA被纳入SP10病毒颗粒中，而病毒和细菌DNA之间没有任何重组。
Bacteriophage SP10 may infect Bacillus subtilis to form a carrier system. The infected bacteria give rise to infected organisms after six or more successive single-colony isolations. About 60% of the spores derived from such an infected culture yield clones that produce phage, and such spores retain their ability to produce phage even after treatment with antiserum and heating at 80[degree]. Four hours'' incubation of carrier spores in nutrient broth yields cultures having more than 10 infective centers per bacterium. Cultivation of infected organisms in medium containing SP10 antiserum leads to loss of phage and loss of immunity to phage infection. DNA with a density characteristic of viral DNA can be detected in infected bacteria. Extracts of infected bacteria contain a phage-induced deoxyribonuclease activity which attacks both phage and bacterial DNA in vitro. The phage-bacterial complex is there- fore best described as a carrier state. The DNA of the phage and of B. subtilis are chemically different. Enzymatically prepared complementary virus RNA has no homology with bacterial DNA. The lack of homology of this transducing phage DNA with the DNA of its host and the failure to form a truly lysogenic relationship reinforce previous conclusions that transducing bacterial DNA is incorporated into the SP10 virus particle without any recombination between viral and bacterial DNA.