Dietary zinc deficiency reduced growth performance, intestinal immune and physical barrier functions related to NF- k B, TOR, Nrf2, JNK and MLCK signaling pathway of young grass carp (Ctenopharyngodon idella)
Dietary zinc deficiency reduced growth performance, intestinal immune and physical barrier functions related to NF- k B, TOR, Nrf2, JNK and MLCK signaling pathway of young grass carp (Ctenopharyngodon idella)
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日粮缺锌降低了幼草鱼（Ctenopharyngodon idella）的生长性能、肠道免疫和与 NF-k B、TOR、Nrf2、JNK 和 MLCK 信号通路相关的物理屏障功能
DOI：
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发表时间：
2017
影响因子：
4.7
通讯作者：
Lin Feng
中科院分区：
文献类型：
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作者：
Zheng-Xing Song;Wei-Dan Jiang;Yang Liu;Pei Wu;Jun Jiang;Xiao-Qiu Zhou;Sheng-Yao Kuang;Ling Tang;Wu-Neng Tang;Yong-An Zhang;Lin Feng
本研究探讨了饲料锌缺乏对幼草鱼生长性能、肠道、免疫和物理屏障功能的影响。630尾草鱼（244.14 ± 0.40 g），分别饲喂不同水平的乳酸锌（10.71、30.21、49.84、72.31、92.56、110.78 mg Zn/kg）和硫酸锌（56.9 mg Zn/kg），试验期60 d。在饲养试验结束时，用嗜水气单胞菌攻击鱼14天。这些结果表明，与适宜锌水平相比，（10.71 mg/kg饲料）减少幼草鱼三个肠段中抗细菌化合物的产生，上调与核因子kappa B（NF- k B）相关的促炎细胞因子，并下调与雷帕霉素靶点（TOR）相关的抗炎细胞因子（P < 0.05），表明饲料锌缺乏可损害鱼类肠道免疫屏障;降低与NF-κ B 2相关因子2（Nrf 2）相关的抗氧化酶的活性和mRNA水平，上调caspase-3、-7、-8的mRNA水平，-9与p38丝裂原活化蛋白相关（p38 MAPK）和c-Jun N-末端蛋白激酶（JNK），在草鱼幼鱼的三个肠段中，与肌球蛋白轻链激酶（MLCK）相关的紧密连接复合物（TJs）的mRNA表达水平明显下降（P < 0.05），说明饲料锌缺乏可损伤鱼类肠道物理屏障。此外，以乳酸锌为锌源，草鱼幼鱼对锌的需要量（以体重增长率、肠炎发病率、近端肠酸性磷酸酶（ACP）活性和近端肠丙二醛（MDA）含量计）分别为61.2、61.4、69.2和69.5mg/kg饲料。根据特定生长率（SGR）、饲料转化率（FE）和对草鱼幼鱼肠炎病的防治效果，乳酸锌的防治效果分别为硫酸锌的132.59%、135.27%和154.04%。
Our study investigated the effects of dietary zinc (Zn) deficiency on growth performance, intestinal.immune and physical barrier functions of young grass carp (Ctenopharyngodon idella). A total of 630 grass.carp (244.14 ± 0.40 g) were fed graded levels of zinc lactate (10.71, 30.21, 49.84, 72.31, 92.56, 110.78 mg.Zn/kg diet) and one zinc sulfate group (56.9 mg Zn/kg diet) for 60 days. At the end of the feeding trial,.fish were challenged with Aeromonas hydrophila for 14 days. These results indicated that compared with.optimal dietary Zn level, dietary Zn deficiency (10.71 mg/kg diet) decreased the production of antibac-.terial compounds, up-regulated pro-inflammatory cytokines related to nuclear factor kappa B (NF- k B).and down-regulated anti-inflammatory cytokines related to target of rapamycin (TOR) in three intestinal.segments of young grass carp (P < 0.05), suggesting that dietary Zn deficiency could impair intestinal.immune barrier of fish; decreased the activities and mRNA levels of antioxidant enzymes related to NF-.E2-related factor 2 (Nrf2), up-regulated the mRNA levels of caspase-3, -7, -8, -9 related to p38 mitogen.activated protein (p38 MAPK) and c-Jun N-terminal protein kinase (JNK), down-regulated the mRNA.levels of tight junction complexes (TJs) related to myosin light chain kinase (MLCK) in three intestinal.segments of young grass carp (P < 0.05), demonstrating that dietary Zn deficiency could injury intestinal.physical barrier of fish. Besides, the Zn requirements (zinc lactate as Zn source) based on percent weight.gain (PWG), against enteritis morbidity, acid phosphatase (ACP) activity in the proximal intestine (PI) and.malondialdehyde (MDA) content in the PI of young grass carp was estimated to be 61.2, 61.4, 69.2 and.69.5 mg/kg diet, respectively. Finally, based on specific growth rate (SGR), feed efficiency (FE) and against.enteritis morbidity of young grass carp, the efficacy of zinc lactate relative to zinc sulfate were 132.59%,.135.27% and 154.04%, respectively.