PREVALENCE AND CHARACTERISTICS OF ESCHERICHIA COLI AND SALMONELLA SPP. IN THE FECES OF WILD URBAN NORWAY AND BLACK RATS (RATTUS NORVEGICUS AND RATTUS RATTUS) FROM AN INNER-CITY NEIGHBORHOOD OF VANCOUVER, CANADA
PREVALENCE AND CHARACTERISTICS OF ESCHERICHIA COLI AND SALMONELLA SPP. IN THE FECES OF WILD URBAN NORWAY AND BLACK RATS (RATTUS NORVEGICUS AND RATTUS RATTUS) FROM AN INNER-CITY NEIGHBORHOOD OF VANCOUVER, CANADA
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DOI：
10.7589/2014-09-242
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发表时间：
2015-07-01
影响因子：
1.3
通讯作者：
Patrick, David M.
中科院分区：
文献类型：
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作者：
Himsworth, Chelsea G.;Zabek, Erin;Patrick, David M.
虽然老鼠粪便被广泛怀疑是致病菌的来源，但很少有研究人员研究老鼠粪便病原体。我们调查了大肠杆菌和沙门氏菌的流行情况和特点。挪威的黑鼠（分别为Rattus norvegicus和Rattus rattus）来自加拿大温哥华的城市社区，于2011年9月和2012年8月收集。结肠内容物培养E.大肠杆菌和沙门氏菌属（Salmonella spp.）筛选出7株最常见的产滋贺毒素的大肠杆菌。大肠杆菌（STEC）血清型。对分离株进行了抗菌素耐药性检测，并对沙门氏菌分离株进行了血清分型。我们检测到E。大肠杆菌阳性率为62.7%（397/633）。397株E.大肠杆菌分离株对>= 1种抗菌药物耐药，而17株（4.3%）为多重耐药（包括两株表现出超广谱β-内酰胺酶耐药的分离株）。633只城市大鼠中有10只（1.6%）携带STEC血清型，包括O 145、O 103、O26和O 45。挪威鼠携带E.大肠埃希菌与黑鼠相比，耐药谱和血清型存在地理聚集性。沙门氏菌在3/633（0.5%）只大鼠中检出，包括血清型Derby、印第安纳州和肠炎。与以大鼠为天然宿主的人畜共患病原体（例如，问号钩端螺旋体、伤寒立克次体、首尔病毒），大鼠可能获得E.大肠杆菌和沙门氏菌属（Salmonella spp.）从他们的环境。大鼠作为环境病原体的“海绵”的能力很少得到考虑，这些生物体在大鼠中的生态学和公共卫生意义需要进一步研究。
Although rat feces are widely suspected to be a source of pathogenic bacteria, few investigators have studied fecal pathogens in rats. We investigated the prevalence and characteristics of Escherichia coli and Salmonella spp. in Norway and black rats (Rattus norvegicus and Rat-tus rattus, respectively) from an urban neighborhood of Vancouver, Canada, collected September 2011 August 2012. Colon content was cultured for E. coli and Salmonella spp. and screened for the seven most-common enteropathogenic Shiga toxin producing E. coli (STEC) serotypes by PCR. Isolates were tested for antimicrobial resistance and Salmonella isolates were sero typed. We detected E. coli in 397/633 (62.7%) urban rats. Forty-one of 397(6.5%) E. coli isolates were resistant to >= 1 antimicrobial while 17 (4.3%) were multidrug resistant (including two isolates demonstrating extended-spectrum beta-lactamase resistance). Ten of 633 (1.6%) urban rats were carrying STEC serotypes including O145, O103, O26, and O45. Norway rats were more likely to be carrying E. coli compared to black rats, and there was geographic clustering of specific resistance patterns and STEC serotypes. Salmonella spp. were detected in 3/633 (0.5%) rats including serotypes Derby, Indiana, and Enteritidis. In contrast to zoonotic pathogens for which rats are the natural reservoir (e.g., Leptospira interrogans, Rickettsia typhi, Seoul virus), rats likely acquired E. coli and Salmonella spp. from their environment. The ability of rats to be a 'sponge' for environmental pathogens has received little consideration, and the ecology and public health significance of these organisms in rats requires further investigation.