Differential expression of microRNAs in Marek's disease virus-transformed T-lymphoma cell lines.
Differential expression of microRNAs in Marek's disease virus-transformed T-lymphoma cell lines.
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DOI：
10.1099/vir.0.009902-0
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
Yongxiu Yao;Yuguang Zhao;Lorraine P. Smith;C. Lawrie;N. Saunders;M. Watson;V. Nair
中科院分区：
文献类型：
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作者：
Yongxiu Yao;Yuguang Zhao;Lorraine P. Smith;C. Lawrie;N. Saunders;M. Watson;V. Nair
microRNA（miRNAs）在不同生物事件中的基因表达调控中发挥着重要作用，包括许多散发性癌症。然而，尽管几种病毒参与诱导癌症，但仅进行了有限数量的研究来检查病毒诱导的瘤形成中的miRNA表达特征，特别是在疱疹病毒诱导的肿瘤中，其中病毒编码的miRNA也对肿瘤细胞的miRNA组有显著贡献。马立克氏病（MD）是由高度传染性的马立克氏病病毒（MDV）诱发的家禽自然发生的快速发病的CD 4 + T细胞淋巴瘤。在MDV转化的淋巴母细胞样细胞系MSB-1中证实了病毒编码的miRNA的高水平表达和几种宿主编码的miRNA的改变的表达。为了鉴定MDV转化细胞特异性的miRNA表达特征，我们通过微阵列分析检测了七种不同MDV转化细胞系中的总体miRNA表达谱。这项研究表明，除了高水平的MDV编码的miRNA外，这些MD肿瘤衍生的淋巴母细胞样细胞系显示出几种宿主编码的miRNA的表达改变。这些细胞系与MDV阴性逆转录病毒转化的AVOL-1细胞系的miRNA表达谱的比较显示，miR-150和miR-223下调，而miR-155的下调是MDV转化的肿瘤细胞特异性的，与病毒病因无关。因此，MDV编码的miRNA的表达增加以及miR-155的特异性下调可以被认为是MD肿瘤细胞的独特表达特征。这些miRNAs的功能靶点的分析将有助于理解MD致癌的分子途径。
MicroRNAs (miRNAs) are increasingly recognized to play crucial roles in regulation of gene expression in different biological events, including many sporadic forms of cancer. However, despite the involvement of several viruses in inducing cancer, only a limited number of studies have been carried out to examine the miRNA expression signatures in virus-induced neoplasia, particularly in herpesvirus-induced tumours where virus-encoded miRNAs also contribute significantly to the miRNome of the tumour cell. Marek's disease (MD) is a naturally occurring, rapid-onset CD4+ T-cell lymphoma of poultry, induced by the highly contagious Marek's disease virus (MDV). High levels of expression of virus-encoded miRNAs and altered expression of several host-encoded miRNAs were demonstrated in the MDV-transformed lymphoblastoid cell line MSB-1. In order to identify the miRNA expression signature specific to MDV-transformed cells, we examined the global miRNA expression profiles in seven distinct MDV-transformed cell lines by microarray analysis. This study revealed that, in addition to the high levels of MDV-encoded miRNAs, these MD tumour-derived lymphoblastoid cell lines showed altered expression of several host-encoded miRNAs. Comparison of the miRNA expression profiles of these cell lines with the MDV-negative, retrovirus-transformed AVOL-1 cell line showed that miR-150 and miR-223 are downregulated irrespective of the viral aetiology, whereas downregulation of miR-155 was specific for MDV-transformed tumour cells. Thus, increased expression of MDV-encoded miRNAs with specific downregulation of miR-155 can be considered as unique expression signatures for MD tumour cells. Analysis of the functional targets of these miRNAs would contribute to the understanding of the molecular pathways of MD oncogenicity.