GABA and glycine in retinal amacrine cells: combined Golgi impregnation and immunocytochemistry.
GABA and glycine in retinal amacrine cells: combined Golgi impregnation and immunocytochemistry.
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视网膜无长突细胞中的 GABA 和甘氨酸：结合高尔基体浸渍和免疫细胞化学。
DOI：
10.1098/rstb.1993.0161
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Yazulla,S
中科院分区：
文献类型：
--
作者：
Sherry,DM;Yazulla,S
高尔基浸渍无长突细胞在全锥蜥蜴视网膜（Anolis carolinensis）的树突和体细胞的标准的基础上，其特征在于。确定了四个主要细胞类别，包括23种类型：三个非分层，13个单层，五个双层和两个三层类型。四种细胞类型包括两个到四个亚型的基础上分层的树突内丛状层（IPL）。高尔基浸渍强烈有利于单层无长突细胞与细胞体在近端边缘的内核层。形态学分类后，用高尔基体-免疫细胞化学法检测23种无长突细胞中神经递质的含量。检查的假定神经递质包括γ-氨基丁酸（GABA）、甘氨酸（GLY）和天冬氨酸（ASP）。17种细胞类型显示GABA免疫反应性（IR），3种细胞类型显示GLY-IR，4种细胞类型既不显示GABA IR也不显示GLY-IR，没有细胞类型显示ASP IR。常规处理的视网膜上的植入后免疫细胞化学证实了谷氨酸脱羧酶，GABA的合成酶，类似于几个GABA-IR高尔基染色类型的细胞的本地化。植入后免疫细胞化学酪氨酸羟化酶（合成酶的儿茶酚胺）和GABA的连续切片表现出共定位的GABA和儿茶酚胺，可能多巴胺，在一个bisterialized无长突细胞类型。我们的结论是，GABA-IR无长突细胞类型更多，形态异质性比GLY-IR无长突细胞。形态的异质性和GABA-IR和GLY-IR无长突细胞类型的排他性（有一个例外）表明，这两种神经递质在蜥蜴内层视网膜中发挥着多种不同的功能作用。
Golgi-impregnated amacrine cells in the all-cone lizard retina (Anolis carolinensis) were characterized on the bases of dendritic and somatic criteria. Four major cell categories, comprising 23 types were identified: three non-stratified, 13 monostratified, five bistratified, and two tristratified types. Four of the cell types comprised two to four subtypes based on stratification of their dendrites within the inner plexiform layer (IPL). Golgi impregnation strongly favoured monostratified amacrine cells with cell bodies at the proximal margin of the inner nuclear layer. The neurotransmitter content of each of the 23 amacrine cell types was examined by combined Golgi-immunocytochemistry after morphological classification. Putative neurotransmitters examined included gamma-aminobutyric acid (GABA), glycine (GLY) and aspartate (ASP). Seventeen cell types showed GABA-immunoreactivity (IR), three cell types showed GLY-IR, and four cell types showed neither GABA-IR nor GLY-IR. No cell types showed ASP-IR. Each cell type had a characteristic neurochemical signature, with the exception of one monostratified cell type that showed three different neurochemical signatures. Postembedding immunocytochemistry on conventionally processed retinas confirmed the localization of glutamic acid decarboxylase, the synthetic enzyme for GABA, to cells similar to several of the GABA-IR Golgi-stained types. Postembedding immunocytochemistry for tyrosine hydroxylase (the synthetic enzyme for catecholamines) and GABA on serial sections demonstrated colocalization of GABA and a catecholamine,probably dopamine, in a bistratified amacrine cell type. We conclude that GABA-IR amacrine cell types are more numerous and morphologically heterogeneous than GLY-IR amacrine cells. The morphological heterogeneity and, with one exception, exclusivity of GABA-IR and GLY-IR amacrine cell types indicate that both neurotransmitters play a variety and different functional roles in the lizard inner retina.