Identification of differentially expressed, tumor-associated proteins in oral squamous cell carcinoma by proteomic analysis
Identification of differentially expressed, tumor-associated proteins in oral squamous cell carcinoma by proteomic analysis
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DOI：
10.1002/elps.200500510
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发表时间：
2006-04-01
期刊：
影响因子：
2.9
通讯作者：
Fountoulakis, M
中科院分区：
文献类型：
--
作者：
Turhani, D;Krapfenbauer, K;Fountoulakis, M
口腔鳞状细胞癌是一种预后较差的恶性肿瘤，早期发现鉴别恶性细胞和正常细胞的标志物对临床诊断具有重要意义。用2-DE和MALDI-TOF-MS分析口腔鳞状细胞癌（OSCC）和对照样品中富含线粒体和细胞质蛋白的亚细胞组分。20种蛋白在OSCC中表达改变；与对照样品相比，14个上调，6个下调。II蛋白、cofilin、c反应蛋白前体、肌酸激酶m链、脂肪酸结合蛋白、角蛋白II型、肌球蛋白轻链2和3、核苷二磷酸激酶A、磷酸甘油酸变化酶1、血小板球蛋白、视黄酸结合蛋白II在OSCC中首次出现差异表达。高度上调水平的蛋白可能是潜在的诊断标记物，因此具有临床意义。
Oral squamous cellular carcinoma is a malignant tumor with poor prognosis and therefore the discovery of early markers to discriminate malignant from normal cells would be of critical importance in clinical diagnosis. Subcellular fractions from oral squamous cell carcinoma (OSCC) and control samples, enriched in mitochondrial and cytosolic proteins, were analyzed by 2-DE, followed by MALDI-TOF-MS. Twenty proteins showed altered expression levels in OSCC; 14 were up- and 6 were down-regulated in comparison with the control samples. For II proteins, cofilin, C-reactive protein precursor, creatine kinase m-chain, fatty acid-binding protein, keratin type II, myosin light chain 2 and 3, nucleoside diphosphate kinase A, phosphoglycerate mutase 1, plakoglobulin, and retinoic acid-binding protein II, it is shown for the first time that they are differentially expressed in OSCC. Proteins with highly up-regulated levels may be of interest as potential diagnostic markers and consequently of clinical interest.