TRANSFORMING GROWTH-FACTOR-BETA (TGF-BETA) IN HUMAN-MILK
TRANSFORMING GROWTH-FACTOR-BETA (TGF-BETA) IN HUMAN-MILK
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DOI：
10.1111/j.1365-2249.1993.tb06004.x
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发表时间：
1993-10-01
影响因子：
4.6
通讯作者：
TSUJII, T
中科院分区：
文献类型：
--
作者：
SAITO, S;YOSHIDA, M;TSUJII, T
通过NRK 47 F细胞的殖民地形成来研究人乳清中所含的TGF-β的量。注意到诱导集落形成的因子确实存在于人乳清中，并且当引入抗TGF-β抗体时，其作用被中和。这表明TGF-β确实存在于人类乳清中。在初乳中，TGF-β的总量为1365-7 +/- 242.9 ng/ml，其中活性形式包括728.1 +/- 248.7 ng/ml（n = 21）。在晚期乳汁中，总TGF-β为952.5 +/- 212.6 ng/ml，活性形式为178.7 +/- 157.3 ng/ml。因此，人乳含有大量的活性TGF-β。此外，通过逆转录聚合酶链反应揭示，编码TGF-β 1和TGF-β 2的mRNA存在于人乳细胞中。这些结果表明，TGF-β 1和TGF-β 2都存在于母乳中。
The amount of TGF-beta contained in human whey was studied by the colony formation of NRK47F cells. It was noted that a factor inducing colony formation did exist in human whey, and its action was neutralized when anti-TGF-beta antibodies were introduced. This suggests that TGF-beta does exist in human whey. In colostrum, the total amount of TGF-beta was 1365-7 +/- 242.9 ng/ml, of which the active form comprised 728.1 +/- 248.7 ng/ml (n = 21). In late milk, the total TGF-beta was 952.5 +/- 212.6 ng/ml, with an active form of 178.7 +/- 157.3 ng/ml. Thus human milk contain's a large amount of active TGF-beta. Furthermore, it was revealed by the reverse transcriptase polymerase chain reaction that mRNAs coding TGF-beta1 and TGF-beta2 exist in human milk cells. These results suggest that both TGF-beta1 and TGF-beta2 exist in human milk.