Basic fibroblast growth factor and granulocyte colony-stimulating factor enhance mucosal surface expansion after adult small bowel transplantation without vascular reconstruction in rats
Basic fibroblast growth factor and granulocyte colony-stimulating factor enhance mucosal surface expansion after adult small bowel transplantation without vascular reconstruction in rats
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DOI：
10.1016/j.jpedsurg.2005.12.019
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发表时间：
2006-04-01
影响因子：
2.4
通讯作者：
Miyano, Takeshi
中科院分区：
文献类型：
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作者：
Lee, Kyeong Deok;Yamataka, Atsuyuki;Miyano, Takeshi
目的：我们先前的研究表明，成年大鼠小肠移植可以成功，而不需要通过去除移植物浆膜进行血管重建。在这项研究中，我们评估，如果粒细胞集落刺激因子（G-CSF）或碱性成纤维细胞生长因子（bFGF）可以提高移植物存活率在同一大鼠model.Method：一个10分钟长的成年小肠移植从一个成年12周龄的刘易斯大鼠被移植到一个小袋中创建的网膜的5周龄的刘易斯大鼠（同基因肠移植[SBTx]，n = 49）。浆膜切除组（n = 29）在SBTx前切除移植物浆膜，非浆膜切除组（n = 20）保留完整。每组又分为3个亚组（sG-1）：sG-2每天皮下注射G-CSF; sG-3在网膜移植物周围持续输注bFGF。所有移植物在SBTx后14天收获并进行组织学研究。使用粘膜表面扩张评分（MSES），其中0 =移植物上无粘膜，1 =四分之一移植物上有粘膜，2 =一半移植物上有粘膜，3 =四分之三移植物上有粘膜，4 =整个移植物上有粘膜。结果：浆膜切除组MSES明显高于非浆膜切除组，表明移植物存活（P <0.0001）。浆膜切除术组粘膜亚组的CD 34阳性毛细血管分别为103.9 +/- 34.2、130.2 +/- 52.0和132.3 +/- 37.7;肌肉亚组分别为74.4 +/- 38.0、86.2 +/- 32.9和82.4 +/- 30.3;网膜分别为73.8 ± 30.1、151.3 ± 60.3和140.0 ± 49.0。sG-2和sG-3的粘膜表面扩张评分和总体CD 34阳性毛细血管显著高于sG-1（均P <0.05）。结论：我们的结果表明G-CSF和bFGF增强血管生成和粘膜表面扩张。(c)2006年爱思唯尔公司All rights reserved.
Aim: We showed previously that adult small bowel could be transplanted successfully in rats without vascular reconstruction by removing the graft serosa. In this study, we assessed if granulocyte colony-stimulating factor (G-CSF) or basic fibroblast growth factor (bFGF) could improve graft survival in the same rat model.Method: A 10-min-long adult small bowel graft from an adult 12-week-old Lewis rat was transplanted into a pouch created in the omentum of a 5-week-old Lewis rat (syngeneic bowel transplantation [SBTx], n = 49). Graft serosa was removed just before SBTx in the serosectomy group (n = 29) and left intact in the nonserosectomy group (n = 20). Each group was divided into 3 subgroups (sG): sG-1 had no G-CSF or bFGF; sG-2 had daily subcutaneous injections of G-CSF; and sG-3 had continuous infusion of bFGF around the graft in the omentum. All grafts were harvested 14 days after SBTx and studied histologically. A mucosal surface expansion score (MSES) was used where 0 = no mucosa on the graft, 1 = mucosa on one fourth of the graft, 2 = mucosa on one half of the graft, 3 = mucosa on three fourths of the graft, and 4 = mucosa on the whole graft. The density of CD34-positive capillaries per 1000 nuclei was also measured.Results: Serosectomy group MSES were significantly higher than nonserosectomy group MSES indicating that grafts survived (P < .0001). CD34-positive capillaries in serosectomy group subgroups for mucosa were 103.9 +/- 34.2, 130.2 +/- 52.0, and 132.3 +/- 37.7, respectively; for muscle, 74.4 +/- 38.0, 86.2 +/- 32.9, and 82.4 +/- 30.3, respectively; and for omentum, 73.8 +/- 30.1, 151.3 +/- 60.3, and 140.0 +/- 49.0, respectively. Mucosal surface expansion score and overall CD34-positive capillaries for sG-2 and sG-3 were significantly higher than for sG-1 (both, P < .05).Conclusion: Our results suggest that G-CSF and bFGF enhance angiogenesis and mucosal surface expansion. (c) 2006 Elsevier Inc. All rights reserved.