Two mouse piwi-related genes:: miwi and mili
Two mouse piwi-related genes:: miwi and mili
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DOI：
10.1016/s0925-4773(01)00499-3
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发表时间：
2001-10-01
影响因子：
2.6
通讯作者：
Nakano, T
中科院分区：
文献类型：
--
作者：
Kuramochi-Miyagawa, S;Kimura, T;Nakano, T
属于piwi家族的基因是多种生物体中干细胞自我更新所必需的。我们克隆小鼠同源的piwi的RT-PCR使用退化引物。小鼠同源物MIWI和MILI的推导的氨基酸序列表明，每个都包含一个保守的C-末端PIWI结构域，每个共享显着的同源性PIWI和他们的人类对应物HIWI。原位杂交结果表明，miwi和mili基因均存在于成年人睾丸生殖细胞中，提示这两个基因可能在精子发生中起作用。此外，mili在发育中的小鼠胚胎的原始生殖细胞（PGCs）中表达，因此可能在生殖细胞形成过程中发挥作用。MIWI可能参与RNA加工或翻译调节，因为发现MIWI具有RNA结合活性。我们的数据表明，miwi和mili调节精子发生和原始生殖细胞的生产。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Genes belonging to the piwi family are required for stem cell self-renewal in diverse organisms. We cloned mouse homologues of piwi by RT-PCR using degenerative primers. The deduced amino acid sequences of mouse homologues MIWI and MILI showed that each contains a well-conserved C-terminal PIWI domain and that each shares significant homology with PIWI and their human counterparts HIWI. Both miwi and mili were found in germ cells of adult testis by in situ hybridization, suggesting that these genes may function in spermatogenesis. Furthermore, mili was expressed in primordial germ cells (PGCs) of developing mouse embryos and may therefore play a role during germ cell formation. MIWI may be involved in RNA processing or translational regulation, since MIWI was found to possess RNA binding activity. Our data suggest that miwi and mili regulate spermatogenesis and primordial germ cell production. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.