Nanogold based electrochemical sensor for determination of norepinephrine in biological fluids
Nanogold based electrochemical sensor for determination of norepinephrine in biological fluids
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DOI：
10.1016/j.snb.2010.10.041
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发表时间：
2011-03
影响因子：
8.4
通讯作者：
R. Goyal;M. Aziz;M. Oyama;Sanghamitra Chatterjee;A. S. Rana
中科院分区：
文献类型：
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作者：
R. Goyal;M. Aziz;M. Oyama;Sanghamitra Chatterjee;A. S. Rana
在氧化铟锡（ITO）表面制备了一层稳定的金纳米粒子（AuNPs），并将其作为电极，采用方波伏安法（SWV）和循环伏安法（CV）对去甲肾上腺素（NE）进行检测。该修饰电极表现出强有力的和持久的电子介导行为，并观察到一个明确的氧化峰对NE。与裸ITO和裸金电极相比，观察到NE的峰电位在较小的正电位处，峰电流增加。在pH7.2的磷酸盐缓冲溶液（PBS）中，在-0.6 ~0.8V的电位范围内，NE在金纳米粒子修饰的ITO上呈现两个准可逆电对。在最佳实验条件下，去甲肾上腺素的浓度在100 ~ 25μM范围内对催化氧化峰电流有响应。检测限为87 nM。此外，pH值的影响表明，NE在金纳米粒子修饰的ITO上的氧化涉及相等数量的质子和电子的转移。还报告了血液和尿液中常见共存代谢物的干扰作用。该修饰电极具有良好的稳定性和重现性。与高效液相色谱法（HPLC）的结果比较表明，一个很好的协议。
A stable layer of gold nano particles (AuNPs) was deposited on the surface of indium tin oxide (ITO) and was used as an electrode for the detection of norepinephrine (NE) by square wave voltammetry (SWV) and cyclic voltammetry (CV). This modified electrode exhibits potent and persistent electron-mediating behavior and a well-defined oxidation peak towards NE was observed. The peak potential of NE was observed at less positive potential with increase in peak current as compared to bare ITO and bare gold electrodes. NE exhibited two quasi-reversible couples at AuNPs modified ITO over the potential range from −0.6 to 0.8V in phosphate buffer solution (PBS) (pH 7.2). At optimal experimental condition, the catalytic oxidative peak current was responsive with the NE concentrations ranging from 100nM to 25μM. The detection limit was found to be 87nM. Also, the effect of pH revealed that the oxidation of NE at the AuNPs modified ITO involved the transfer of equal number of protons and electrons. The interfering effect of common coexisting metabolites in blood and urine has also been reported. The modified electrode exhibited high stability and reproducibility. A comparison of results with high performance liquid chromatography (HPLC) signalizes a good agreement.