THE PROMOTER OF TL-DNA GENE 5 CONTROLS THE TISSUE-SPECIFIC EXPRESSION OF CHIMERIC GENES CARRIED BY A NOVEL TYPE OF AGROBACTERIUM BINARY VECTOR
THE PROMOTER OF TL-DNA GENE 5 CONTROLS THE TISSUE-SPECIFIC EXPRESSION OF CHIMERIC GENES CARRIED BY A NOVEL TYPE OF AGROBACTERIUM BINARY VECTOR
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DOI：
10.1007/bf00331014
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
SCHELL, J
中科院分区：
文献类型：
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作者：
KONCZ, C;SCHELL, J
构建了与各种大肠杆菌基因克隆载体组合使用的“植物基因载体盒”。该盒包含一个复制和动员单元，使其能够在大肠杆菌之间维持和来回转移。大肠杆菌和根癌农杆菌宿主，条件是这些宿主含有质粒RK 2复制和动员辅助功能。该盒还含有可转移的DNA单元，其具有植物选择标记基因和可与不同细菌复制子组合的克隆位点，从而便于在大肠杆菌中从转化植物中重新分离转移的DNA。杆菌该载体盒含有两种不同的启动子，它们来源于T-DNA编码的基因5和胭脂碱合酶（NOS）。通过比较与这些启动子序列中的每一个连接的标记酶的表达水平，发现基因5启动子以组织特异性方式是有活性的，而NOS启动子的情况并非如此。这一观察结果提供了第一个记录的实例，从原核宿主的基因，其表达显然是由植物生长因子调节。
A "plant gene vector cassette" to be used in combination with various Escherichia coli gene-cloning vectors was constructed. This cassette contains a replication and mobilization unit which allows it to be maintained and to be transferred back and forth between E. coli and Agrobacterium tumefaciens hosts provided these hosts contain plasmid RK2 replication and mobilization helper functions. The cassette also harbors a transferable DNA unit with plant selectable marker genes and cloning sites which can be combined with different bacterial replicons, thus facilitating the reisolation of transferred DNA from transformed plants in E. coli. The vector cassette contains two different promoters derived from the T-DNA-encoded genes 5 and nopaline synthase (NOS). By comparing the levels of expression of the marker enzymes linked to each of these promoter sequences, it was found that the gene 5 promoter is active in a tissue-specific fashion whereas this is not the case for the NOS promoter. This observation provides the first documented instance of a gene from a procaryotic host the expression of which is apparently regulated by plant growth factors.