Chromosome analyses of human tumours following hetero-transplantation to the mouse mutant nude.
Chromosome analyses of human tumours following hetero-transplantation to the mouse mutant nude.
复制标题
异种移植到小鼠突变体裸体后对人类肿瘤进行染色体分析。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
J. Rygaard
中科院分区：
文献类型：
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作者：
J. Visfeldt;C. Povlsen;J. Rygaard
本研究的目的是研究移植到患有胸腺发育不全的突变裸小鼠后，一些实体人类肿瘤的染色体构成。发现裸鼠具有正常的鼠核型。在肿瘤组织上进行染色体分析，使用直接方法而无需先前培养。最重要的研究结果是只发现了具有人型染色体的细胞，所分析的大多数细胞为超二倍体，染色体数目存在明显的变异。在某些情况下，可以将肿瘤连续移植到裸鼠体内。在早期和晚期传代的肿瘤细胞中，只发现含有人类染色体的核型，因此，在所采用的异源移植系统中没有发现杂交或总的染色体种类转移。
The purpose of the present investigation was to study the chromosomal constitution in a number of solid human tumours after transplantation to the mouse mutant nude, suffering from thymic aplasia. The nude mouse was found to have a normal murine karyotype. The chromosome analyses were made on tumour tissue, using a direct method without previous cultivation. The most important result of the investigation is that only cells with chromosomes of human type were found. The majority of the cells analyzed were hyperdiploid, presenting an appreciable variation in the chromosome numbers. In some cases it was possible to transplant the tumours in the nude mice serially. In tumour cells both from early and late passages, only karyotypes which contained nothing but human chromosomes were found. Hence, no hybridization or total chromosomal species shift was revealed in the heterotransplantation system employed.