Some characteristics of processing sites in ribosomal precursor RNA of yeast
Some characteristics of processing sites in ribosomal precursor RNA of yeast
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酵母核糖体前体RNA加工位点的一些特征
DOI：
10.1093/nar/8.13.2907
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发表时间：
1980
影响因子：
14.9
通讯作者：
R. Planta
中科院分区：
文献类型：
--
作者：
G. Veldman;R. Brand;J. Klootwijk;R. Planta
测定了酵母中核糖体RNA操纵子17S和5.8S rRNA基因间隔区的DNA序列。在这个区域，37S核糖体前体RNA在成熟过程中的一些位置被特异性切割。通过对各个RNA物种的末端片段的序列分析，已经确定了这些加工位点的确切位置。18S次级前体RNA两端周围的序列之间以及17S成熟rRNA两端周围的序列之间似乎没有显著的互补性。这一发现意味着酵母37S核糖体前体RNA的加工不是由先前被证明参与大肠杆菌核糖体前体RNA加工的双链特异性核糖核酸酶指导的[见参考文献1，2]。本论文中描述的酵母核糖体前体RNA的加工位点都在一侧被非常富含[A+T]的序列所包围。此外，在该前体RNA的加工位点周围发现了序列重复。最后，在许多成熟rRNA产物和中间核糖体RNA前体的3‘-末端[6个核苷酸]和5’-末端[13个核苷酸]存在序列同源性。根据加工酶可能的识别位点讨论了这些结构特征。
The DNA sequences of the intergenic region between the 17S and 5.8S rRNA genes of the ribosomal RNA operon in yeast has been determined. In this region the 37S ribosomal precursor RNA is specifically cleaved at a number of sites in the course of the maturation process. The exact position of these processing sites has been established by sequence analysis of the terminal fragments of the respective RNA species. There appears to be no significant complementarity between the sequences surrounding the two termini of the 18S secondary precursor RNA nor between those surrounding the two termini of 17S mature rRNA. This finding implies that the processing of yeast 37S ribosomal precursor RNA is not directed by a double-strand specific ribonuclease previously shown to be involved in the processing of E. coli ribosomal precursor RNA [see Refs 1,2]. The processing sites of yeast ribosomal precursor RNA described in the present paper are all flanked at one side by a very [A+T]-rich sequence. In addition, sequence repeats are found around the processing sites in this precursor RNA. Finally, sequence homologies are present at the 3'-termini [6 nucleotides] and the 5'-termini [13 nucleotides] of a number of mature rRNA products and intermediate ribosomal RNA precursors. These structural features are discussed in terms of possible recognition sites for the processing enzymes.