PROTECTION BY FREE OXYGEN RADICAL SCAVENGING ENZYMES AGAINST GLUCOSE-INDUCED EMBRYONIC MALFORMATIONS INVITRO
PROTECTION BY FREE OXYGEN RADICAL SCAVENGING ENZYMES AGAINST GLUCOSE-INDUCED EMBRYONIC MALFORMATIONS INVITRO
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DOI：
10.1007/bf00405004
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发表时间：
1991-05-01
期刊：
影响因子：
8.2
通讯作者：
BORG, LAH
中科院分区：
文献类型：
--
作者：
ERIKSSON, UJ;BORG, LAH
本研究探讨了糖尿病妊娠的致畸作用与胚胎氧自由基活性增加有关的可能性。大鼠胚胎在50 mmol/l葡萄糖中培养48 h，随后表现出明显的生长迟缓和严重畸形。酶诱导剂Citiolone和自由氧自由基清除酶超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶保护免受干扰的生长和发育的胚胎在50 mmol/L的葡萄糖时，加入到培养基中。酶的测量表明，西硫龙诱导的胚胎组织中的超氧化物歧化酶的活性增加，并采取了卵黄囊和胚胎适当的酶。因此，通过增加胚胎组织的自由氧自由基清除能力的试剂赋予对胚胎发育不良的保护。结果表明，高浓度的葡萄糖在体外引起胚胎畸形通过产生氧自由基。胚胎组织中这种自由基的产生增加可能与糖尿病妊娠中先天性畸形的风险增加直接相关。
This study addresses the possibility that the teratogenic effects of a diabetic pregnancy are associated with increased embryonic activities of free oxygen radicals. Rat embryos were cultured in 50 mmol/l glucose for 48 h and subsequently showed pronounced growth retardation and severe malformations. The enzyme inducer citiolone and the free oxygen radical scavenging enzymes superoxide dismutase, catalase and glutathione peroxidase protected against the disturbed growth and development of the embryos at 50 mmol/l glucose when added to the culture media. Enzymatic measurements indicated that citiolone induced an increased activity of superoxide dismutase in the embryonic tissues and that the added enzymes were taken up by both the yolk sac and the embryo proper. The protection against embryonic maldevelopment was thus conferred by agents that increased the free oxygen radical scavenging capacity of the embryonic tissues. The results suggest that a high glucose concentration in vitro causes embryonic dysmorphogenesis by generation of free oxygen radicals. An enhanced production of such radicals in embryonic tissues may be directly related to the increased risk of congenital malformations in diabetic pregnancy.