A genetic linkage map of the mouse using an expanded production system of restriction landmark genomic scanning (RLGS Ver.1.8).
A genetic linkage map of the mouse using an expanded production system of restriction landmark genomic scanning (RLGS Ver.1.8).
复制标题
使用限制性标志基因组扫描的扩展生产系统（RLGS Ver.1.8）绘制小鼠的遗传连锁图。
DOI：
10.1006/bbrc.1994.2895
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发表时间：
1994
影响因子：
3.1
通讯作者：
Kitajima,M
中科院分区：
文献类型：
--
作者：
Okazaki,Y;Okuizumi,H;Sasaki,N;Ohsumi,T;Kuromitsu,J;Kataoka,H;Muramatsu,M;Iwadate,A;Hirota,N;Kitajima,M
We have developed an expanded system (RLGS Ver.1.8) for producing RLGS patterns that result in a 16-fold increase in the number of gels produced and a 10-fold reduction in the total cost per gel. The major modifications include: 1) performing the blocking and labeling step without phenol extraction or ethanol precipitation; 2) minimizing the reaction volume and the enzyme units in each step; 3) developing a long vertical agarose disc gel electrophoresis for the 1st-dimension; and 4) developing a new apparatus for multiplex vertical 2nd-dimensional electrophoresis. RLGS Ver.1.8 was used with a new combination of restriction enzymes to identify variation for 209 loci between C57BL/6J and DBA/2J. Twenty-six BXD RI strains were analyzed and 195/209 loci were genetically mapped. These loci were mapped in one week of laboratory work by two people. This system provides an important tool for the genetic analysis of new loci in similar genetic resources.
