Photoblueing of organic dyes can cause artifacts in super-resolution microscopy
Photoblueing of organic dyes can cause artifacts in super-resolution microscopy
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DOI：
10.1038/s41592-021-01061-2
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发表时间：
2021-02-25
期刊：
影响因子：
48
通讯作者：
Sauer, Markus
中科院分区：
文献类型：
--
作者：
Helmerich, Dominic A.;Beliu, Gerti;Sauer, Markus
荧光团的照射可以引起荧光能力的丧失，称为光漂白。有趣的是，一些荧光团光转换为蓝移荧光分子作为光漂白途径上的中间体，这可能使荧光成像复杂化，特别是在超分辨率荧光成像中使用的强激光照射下。在这里，我们讨论了荧光团的光致蓝化的机制及其对荧光成像的影响，并展示了如何防止它。
Illumination of fluorophores can induce a loss of the ability to fluoresce, known as photobleaching. Interestingly, some fluorophores photoconvert to a blue-shifted fluorescent molecule as an intermediate on the photobleaching pathway, which can complicate multicolor fluorescence imaging, especially under the intense laser irradiation used in super-resolution fluorescence imaging. Here, we discuss the mechanisms of photoblueing of fluorophores and its impact on fluorescence imaging, and show how it can be prevented.