A novel role for GM-CSF: enhancement of pregnancy specific interferon production, ovine trophoblast protein-1.
A novel role for GM-CSF: enhancement of pregnancy specific interferon production, ovine trophoblast protein-1.
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GM-CSF 的新作用：增强妊娠特异性干扰素、绵羊滋养层蛋白-1 的产生。
DOI：
10.1210/endo.132.4.7681767
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发表时间：
1993
期刊：
影响因子：
4.8
通讯作者：
R. Christenson
中科院分区：
文献类型：
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作者：
K. Imakawa;S. Helmer;K. Nephew;C. S. Meka;R. Christenson
绵羊滋养层蛋白-1（oTP-1）的瞬时和大规模生产的着床前的概念似乎是一个关键的事件所需的建立成功的妊娠。我们以前已经证明，几个oTP-1基因之一，主要是在怀孕13天和20天之间表达，这个oTP-1基因含有一个AP-1位点，转录增强子元件，在5 '侧翼区。我们已经获得的证据表明，细胞因子粒细胞巨噬细胞集落刺激因子（GM-CSF）和滋养层干扰素（IFN），oTP-1的孕体生产之间的联系。这些是：1）通过在体外添加GM-CSF来增强oTP-1产生（多肽和mRNA两者）和2）GM-CSF mRNA定位于子宫内膜的腔上皮和腺上皮中。基于这些观察结果，我们提出，大量的oTP-1在怀孕期间产生的建立至少部分由母体GM-CSF刺激。
Transient and massive production of ovine trophoblast protein-1 (oTP-1) by preimplantation conceptuses seems to be a critical event required for the establishment of successful pregnancy. We have previously demonstrated that one of several oTP-1 genes is predominantly expressed between days 13 and 20 of pregnancy and that this oTP-1 gene contains an AP-1 site, a transcription enhancer element, in the 5'-flanking region. We have obtained evidence, indicating a linkage between cytokine granulocyte macrophage-colony stimulating factor (GM-CSF) and conceptus production of the trophoblast interferon (IFN), oTP-1. These are: 1) oTP-1 production (both polypeptide and mRNA) is enhanced by the addition of GM-CSF in vitro and 2) GM-CSF mRNA is localized in the luminal and glandular epithelium of the uterine endometrium. Based on these observations, we propose that the massive amounts of oTP-1 produced during the period of pregnancy establishment is stimulated at least in part by maternal GM-CSF.