Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides
Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides
复制标题
DOI：
10.1016/j.biortech.2016.01.073
复制
发表时间：
2016-04-01
影响因子：
11.4
通讯作者：
Liu, Jia
中科院分区：
文献类型：
--
作者：
Huang, Xiang-Feng;Liu, Jia-Nan;Liu, Jia
以不同浓度的乙酸为碳源，对环状红孢子虫AS 2.1389进行了低成本制备微生物脂质的试验研究，为生物柴油生产提供了良好的原料。在以4 ~ 20 g/L乙酸为唯一碳源的培养基中，与在相同培养条件下的含葡萄糖培养基中相比，其脂质含量更高。在乙酸浓度为20 g/L、碳氮比（C/N）为200的条件下，生物量产量最高，为4.35 g/L，脂质含量为48.2%。当乙酸浓度低至4 g/L时，C/N为100的顺序分批培养（SBC）可将生物量提高至4.21 g/L，脂质含量为38.6%。这些结果为利用不同的废物衍生的挥发性脂肪酸生产脂质提供了可行的培养策略。(C) 2016 Elsevier Ltd.版权所有。
Rhodosporidium toruloides AS 2.1389 was tested using different concentrations of acetic acid as a low-cost carbon source for the production of microbial lipids, which are good raw materials for biodiesel production. It grew and had higher lipid contents in media containing 4-20 g/L acetic acid as the sole carbon source, compared with that in glucose-containing media under the same culture conditions. At acetic acid concentrations as high as 20 g/L and the optimal carbon-to-nitrogen ratio (C/N) of 200 in a batch culture, the highest biomass production was 4.35 g/L, with a lipid content of 48.2%. At acetic acid concentrations as low as 4 g/L, a sequencing batch culture (SBC) with a C/N of 100 increased biomass production to 4.21 g/L, with a lipid content of 38.6%. These results provide usable culture strategies for lipid production by R. toruloides AS 2.1389 when using diverse waste-derived volatile fatty acids. (C) 2016 Elsevier Ltd. All rights reserved.