Early events elicited by bombesin and structurally related peptides in quiescent Swiss 3T3 cells. II. Changes in Na+ and Ca2+ fluxes, Na+/K+ pump activity, and intracellular pH
Early events elicited by bombesin and structurally related peptides in quiescent Swiss 3T3 cells. II. Changes in Na+ and Ca2+ fluxes, Na+/K+ pump activity, and intracellular pH
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铃蟾肽和结构相关肽在静止的 Swiss 3T3 细胞中引发的早期事件。
DOI：
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发表时间：
1986
影响因子：
7.8
通讯作者：
E. Rozengurt
中科院分区：
文献类型：
--
作者：
S. Mendoza;J. Schneider;A. López;J. Sinnett;E. Rozengurt
两栖动物的十四肽，蛙皮素，和结构相关的肽引起了显着增加哇巴因敏感的86 Rb+摄取（Na+/K+泵活性的措施）在静止的瑞士3 T3细胞。这种作用在加入肽后几秒钟内发生，似乎是通过Na+进入细胞的增加介导的。蛙皮素对Na+内流和Na+/K+泵活性的影响是浓度依赖性的，半最大刺激发生在0.3- 0.4 nM。结构上相关的肽litorin、胃泌素释放肽和神经介肽B也刺激哇巴因敏感的86 Rb+摄取;这些肽刺激Na+/K+泵的相对效力与它们增加DNA合成的效力相当（Zachary，I.，和E. Rozengurt，1985，Proc. Natl. Acad. Sci.美国，82：7616-7620）。蛙皮素增加Na+内流，至少部分，通过Na+/H+反向。该肽增加细胞内pH值，这种效果被取消在细胞外Na+的情况下。除了单价离子转运，蛙皮素和结构相关的肽迅速增加流出的45钙+从静止的瑞士3 T3细胞。该Ca 2+来自细胞内池，并且外排与总细胞内Ca 2+减少50%相关。这些肽还引起胞质游离钙浓度的快速增加。用佛波醇二丁酸酯延长Swiss 3 T3细胞的预处理，这导致蛋白激酶C活性的丧失（Rodriguez-佩纳，A.，和E. Rozengurt，1984，Biochem. Biophys.通信报告：120：1053-1059），极大地降低了蛙皮素对86 Rb+摄取和Na+进入的刺激，暗示该磷酸转移酶系统介导了对蛙皮素的这些应答的一部分。由于在佛波醇二丁酸酯预处理的细胞中观察到蛙皮素对单价离子转运的一些激活，因此很可能该肽还通过第二种机制刺激单价离子转运。
The amphibian tetradecapeptide, bombesin, and structurally related peptides caused a marked increase in ouabain-sensitive 86Rb+ uptake (a measure of Na+/K+ pump activity) in quiescent Swiss 3T3 cells. This effect occurred within seconds after the addition of the peptide and appeared to be mediated by an increase in Na+ entry into the cells. The effect of bombesin on Na+ entry and Na+/K+ pump activity was concentration dependent with half-maximal stimulation occurring at 0.3- 0.4 nM. The structurally related peptides litorin, gastrin-releasing peptide, and neuromedin B also stimulated ouabain-sensitive 86Rb+ uptake; the relative potencies of these peptides in stimulating the Na+/K+ pump were comparable to their potencies in increasing DNA synthesis (Zachary, I., and E. Rozengurt, 1985, Proc. Natl. Acad. Sci. USA., 82:7616-7620). Bombesin increased Na+ influx, at least in part, through an Na+/H+ antiport. The peptide augmented intracellular pH and this effect was abolished in the absence of extracellular Na+. In addition to monovalent ion transport, bombesin and the structurally related peptides rapidly increased the efflux of 45Ca2+ from quiescent Swiss 3T3 cells. This Ca2+ came from an intracellular pool and the efflux was associated with a 50% decrease in total intracellular Ca2+. The peptides also caused a rapid increase in cytosolic free calcium concentration. Prolonged pretreatment of Swiss 3T3 cells with phorbol dibutyrate, which causes a loss of protein kinase C activity (Rodriguez- Pena, A., and E. Rozengurt, 1984, Biochem. Biophys. Res. Commun., 120:1053-1059), greatly decreased the stimulation of 86Rb+ uptake and Na+ entry by bombesin implicating this phosphotransferase system in the mediation of part of these responses to bombesin. Since some activation of monovalent ion transport by bombesin was seen in phorbol dibutyrate- pretreated cells, it is likely that the peptide also stimulates monovalent ion transport by a second mechanism.
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肌醇 1,4,5-三磷酸。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Joseph,SK;Thomas,AP;Williams,RJ;Irvine,RF;Williamson,JR
通讯作者：
Williamson,JR
豚鼠和大鼠脑中胃泌素释放肽相关物质的鉴定。
DOI：
10.1016/0006-291x(83)91497-3
发表时间：
1983
影响因子：
3.1
作者：
Roth,KA;Evans,CJ;Lorenz,RG;Weber,E;Barchas,JD;Chang,JK
通讯作者：
Chang,JK
肌醇三磷酸对胰岛素分泌肿瘤细胞 Ca2+ 通量的影响。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Joseph,SK;Williams,RJ;Corkey,BE;Matschinsky,FM;Williamson,JR
通讯作者：
Williamson,JR
离体肝细胞中肌醇多磷酸的产生与加压素诱导的胞质游离 Ca2+ 增加之间的关系。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Thomas,AP;Alexander,J;Williamson,JR
通讯作者：
Williamson,JR
促细胞分裂剂和蜂毒肽刺激人成纤维细胞中细胞内游离钙浓度的增加。
DOI：
10.1016/0006-291x(84)91619-x
发表时间：
1984
影响因子：
3.1
作者：
Mix,LL;Dinerstein,RJ;Villereal,ML
通讯作者：
Villereal,ML