Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration
Regulation of leg size and shape by the Dachsous/Fat signalling pathway during regeneration
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DOI：
10.1242/dev.035204
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发表时间：
2009-07-01
期刊：
影响因子：
4.6
通讯作者：
Noji, Sumihare
中科院分区：
文献类型：
--
作者：
Bando, Tetsuya;Mito, Taro;Noji, Sumihare
一条被截肢的板球腿再生出了所有失去的部分，具有正常的大小和形状，这表明再生的胚基细胞知道它们的位置和腿的正常大小。然而，调控这一过程的分子机制仍然难以捉摸。在这里，我们使用一个板球模型来表明，Dachsous/脂肪（Ds/Ft）信号通路是必不可少的腿再生。我们发现，敲低ft或ds转录的再生依赖性RNA干扰（rdRNAi）抑制增殖的再生细胞沿着proximodistal（PD）轴伴随着重塑的预先存在的残端，使再生腿短于正常。相比之下，敲除扩增的（ex）或Merlin（Mer）转录物诱导再生细胞的过度增殖，使再生的腿更长。这些结果与在腿移植诱导的中间再生期间使用rdRNAi获得的结果一致。我们提出了一个模型来解释我们的结果，其中的Ds/Ft梯度的陡度控制生长沿着PD轴的再生腿。
An amputated cricket leg regenerates all missing parts with normal size and shape, indicating that regenerating blastemal cells are aware of both their position and the normal size of the leg. However, the molecular mechanisms regulating this process remain elusive. Here, we use a cricket model to show that the Dachsous/Fat (Ds/Ft) signalling pathway is essential for leg regeneration. We found that knockdown of ft or ds transcripts by regeneration-dependent RNA interference (rdRNAi) suppressed proliferation of the regenerating cells along the proximodistal (PD) axis concomitantly with remodelling of the pre-existing stump, making the regenerated legs shorter than normal. By contrast, knockdown of the expanded (ex) or Merlin (Mer) transcripts induced over-proliferation of the regenerating cells, making the regenerated legs longer. These results are consistent with those obtained using rdRNAi during intercalary regeneration induced by leg transplantation. We present a model to explain our results in which the steepness of the Ds/Ft gradient controls growth along the PD axis of the regenerating leg.