MOLECULAR-CLONING AND SEQUENCING OF A CDNA-ENCODING A HUMAN ALPHA(1A) ADRENERGIC-RECEPTOR
MOLECULAR-CLONING AND SEQUENCING OF A CDNA-ENCODING A HUMAN ALPHA(1A) ADRENERGIC-RECEPTOR
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DOI：
10.1016/0006-291x(91)91740-4
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发表时间：
1991-09-30
影响因子：
3.1
通讯作者：
BERELOWITZ, M
中科院分区：
文献类型：
--
作者：
BRUNO, JF;WHITTAKER, J;BERELOWITZ, M
在聚合酶链反应中使用来自大鼠海马cDNA文库的DNA和针对先前克隆的G蛋白受体的保守区域的高度简并寡核苷酸引物组来选择性地扩增和克隆该基因家族的新成员。用PCR扩增的610 bp片段筛选人海马cDNA文库，获得一个cDNA克隆H318/3。该克隆的推导的氨基酸序列编码501个氨基酸的蛋白质，该蛋白质与先前克隆的G蛋白受体具有很强的序列同源性。核苷酸序列分析表明，克隆H318/3与大鼠α 1A肾上腺素能受体的同源性为78%，与第1 ~ 7跨膜区的同源性为95%。基于这种高度的序列同源性，我们得出结论，克隆H318/3代表人α 1A肾上腺素能受体的cDNA。
DNA from a rat hippocampus cDNA library and sets of highly degenerate oligonucleotide primers directed toward conserved regions of previosly cloned G-protein receptors were used in the polymerase chain reaction to selectively amplify and clone new members of this gene family. A human hippocampus cDNA library was screened with a 610 base pair fragment generated by PCR and a cDNA clone, H318/3, was isolated. The deduced amino acid sequence of this clone encoded a protein of 501 amino acids that showed strong sequence homology to previously cloned G-protein receptors. Nucleotide sequence analysis revealed clone H318/3 was 78% homologous to a rat α1Aadrenergic receptor with homology being 95% when comparisons were made in the region that lies between the first to the seventh transmembrane domains. Based on this high degree of sequence homology, we conclude that clone H318/3 represents a cDNA for a human α1Aadrenergic receptor.