Lab on a smartphone (LOS): A smartphone-integrated, plasmonic-enhanced optoelectrowetting (OEW) platform for on-chip water quality monitoring through LAMP assays
Lab on a smartphone (LOS): A smartphone-integrated, plasmonic-enhanced optoelectrowetting (OEW) platform for on-chip water quality monitoring through LAMP assays
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DOI：
10.1016/j.snb.2022.131543
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发表时间：
2022-05
期刊：
影响因子：
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通讯作者：
Si Kuan Thio;Sungwoo Bae;Sung-Yong Park
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文献类型：
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作者：
Si Kuan Thio;Sungwoo Bae;Sung-Yong Park
我们提出了一种低成本的智能手机实验室（LOS）平台，用于快速和现场监测粪便指示菌（FIB），如大肠杆菌(E。大肠杆菌)，以检查环境水域中粪便污染的存在和浓度水平。为了实现LOS，三个主要组件，一个等离子体增强光电润湿（OEW）装置，一个透明加热器和一个智能手机，被紧凑地组装在芯片上，通过环介导的等温放大（LAMP）分析进行水质监测。LOS与OEW设备的集成可以使用等离子体增强的OEW技术进行片上水样制备的无泵和无管液滴操作。与透明加热器的集成提供65°C的等温加热，在LOS平台上方便地实施低成本，便携式LAMP分析。最后，使用智能手机作为集成光学探测器，在LAMP反应过程中拍摄目标水样的数字图像，同时使用智能手机的图像处理应用程序进行时间相关的红绿蓝（RGB）分析，以进行定量和准确的比色研究。实验上，我们已经成功地演示了添加了LAMP的水样的片上样品处理。coliDNA和LAMP混合物，并对e。coliDNA，同时在30分钟内获得LAMP测试结果，然后进行准确的时间依赖性比色评估。LOS平台真正提供了一种低成本，便携式和完全集成的系统，用于快速，现场检测环境水中FIB及其相关病原体的存在，而无需复杂的实验室设备或熟练的人员。
We present a low-cost lab-on-a-smartphone (LOS) platform for rapid and in-situ monitoring of fecal indicator bacteria (FIB) such asEscherichia coli(E. coli) to examine the presence and concentration levels of fecal contamination in environmental waters. To realize the LOS, three main components, a plasmonic-enhanced optoelectrowetting (OEW) device, a transparent heater, and a smartphone, are compactly assembled for on-chip water quality monitoring through loop-mediated isothermal amplification (LAMP) assays. The LOS integration with the OEW device enables pumpless and tubeless droplet manipulations for on-chip water sample preparation using a plasmonic-enhanced OEW technology. The integration with a transparent heater provides isothermal heating at 65 °C, where low-cost, portable LAMP assays are conveniently implemented on the LOS platform. Finally, a smartphone is used as an integrated optical detector to take digital images of target water samples during the LAMP reaction, while a time-dependent red-green-blue (RGB) analysis is performed for quantitative and accurate colorimetric study using a smartphone’s image processing app. Experimentally, we have successfully demonstrated on-chip sample processing of water samples spiked withE. coliDNA and LAMP mixtures, and conducted in-situ isothermal LAMP amplifications ofE. coliDNA, while obtaining the LAMP test results within 30 min, followed by an accurate time-dependent colorimetric assessment. The LOS platform truly offers a low-cost, portable, and fully integrated system for rapid, on-site detection for the presence of FIB and their associated pathogens in environmental water without the need for sophisticated laboratory equipment or skilled personnel.