Epidermal responses of chum salmon (Oncorhynchus keta) fry to the ectoparasitic flagellate Ichthyobodo necator
Epidermal responses of chum salmon (Oncorhynchus keta) fry to the ectoparasitic flagellate Ichthyobodo necator
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鲑鱼 (Oncorhynchus keta) 鱼苗对外寄生鞭毛虫 Ichthyobodo necator 的表皮反应
DOI：
10.1139/z92-216
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
S. Urawa
中科院分区：
文献类型：
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作者：
S. Urawa
用光学显微镜和扫描电镜对鲑鱼鱼苗（Oncorhynchus凯塔）和宿主表皮对感染的反应进行了15周的监测。皮肤上的寄生虫密度在感染后3周达到峰值，平均为625个寄生虫/mm 2，然后在第10周降至接近零。用Alcian蓝（AB，pH2.5）和过碘酸-希夫试剂（PAS）染色程序显示寄生虫感染过程中表皮粘液分泌的程度和组织化学均发生显著变化。粘液细胞的数量显着下降，在前4周的寄生虫密度增加，但此后上升到2000个细胞/mm 2的第8周，当粘液细胞染色PAS单独占主导地位。这种增加是伴随着寄生虫密度的减少而发生的。对照鱼的表皮粘液细胞浓度在同一时期稳定在400 ~ 800个/mm ~ 2，而酸性粘液细胞浓度在同一时期稳定在400 ~ 800个/mm ~ 2。
The numbers of the ectoparasitic flagellate Ichthyobodo necator on chum salmon fry (Oncorhynchus keta) and the host's epidermal responses to infection were monitored for 15 weeks with light and scanning electron microscopy. Parasite density on the skin peaked at 3 weeks postinfection, at an average of 625 parasites/mm2, and then decreased to near zero by week 10. The staining procedure with Alcian blue (AB, pH 2.5) and periodic acid – Schiffs reagent (PAS) revealed dramatic changes in both the degree and histochemistry of epidermal mucus secretion during the course of the parasite infections. The numbers of mucous cells declined significantly during the first 4 weeks as parasite density increased, but thereafter rose to 2000 cells/mm2 by week 8, when mucous cells stained with PAS alone predominated. This increase occurred concomitantly with a reduction in parasite density. In the control fish, the concentration of epidermal mucous cells was stable at 400 – 800 cells/mm2 during the same period and acid muc...