In vivo and in vitro induction of IL-6 by Quillaja saponaria Molina triterpenoid formulations
In vivo and in vitro induction of IL-6 by Quillaja saponaria Molina triterpenoid formulations
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DOI：
10.1006/cyto.1997.0219
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发表时间：
1997-09-01
期刊：
影响因子：
3.8
通讯作者：
VillacresEriksson, M
中科院分区：
文献类型：
--
作者：
Behboudi, S;Morein, B;VillacresEriksson, M
Quillaja Saponaria Molina和一些已定义的Quillaja组分是强有力的佐剂，佐剂的一个重要功能是激活抗原提呈细胞(APC)，这是免疫应答发生的先决条件。白介素6(IL-6)已被认为是产生细胞溶解T细胞的关键因子，它是免疫刺激复合体(ISCOM)携带的抗原诱导的重要效应机制。为了确定与ISCOM独特性质相关的因素来介导CTL应答，我们分析了不同剂型中不同定义的Quillaja三萜组分在体内外刺激APC产生IL-6的能力。用Quillaja佐剂和流感病毒包膜蛋白形成的ISCOM诱导的IL-6的分泌最高，ISCOM的无抗原基质也能刺激IL-6的产生，甚至Quillaja三萜类化合物作为游离成分也能刺激IL-6的产生，但幅度明显较小。在所测试的QH-A和QH-C的各种组合以及原始的半纯化螺苷中，QH-A和QH-C的基质是激活APC产生IL-6的最有效的配方，总的来说，随着QH-A和QH-C比例的增加，激活APC的能力增加，结果表明，同一颗粒中掺入的抗原和佐剂成分对免疫原性具有协同作用。(C)1997年学术出版社有限公司。
Quillaja saponaria Molina and some of the defined Quillaja components are potent adjuvants, An important function of adjuvants is the activation of antigen-presenting cells (APC), a prerequisite for the development of immune responses, Interleukin 6 (IL-6) has been identified as a key factor in the generation of cytolytic T lymphocytes, which constitute an important effector mechanism elicited by immune-stimulatory complex (iscom)-borne antigens, To identify factors relevant to the unique property of iscoms to mediate CTL responses, we analysed the capacity of different defined Quillaja triterpenoid components in various formulations to stimulate production of IL-6 by APC in vitro and in vivo. The iscom formed with Quillaja adjuvant and incorporated influenza virus envelope proteins elicited the highest secretion of IL-6, The production of IL-6 was also stimulated by the antigen free matrix of the iscom and even by the Quillaja triterpenoids as free components albeit to a significantly lesser extent. Among the various combinations of QH-A and QH-C tested and also the original semipurified spikoside, the QH 7.0.3 matrix was the most efficient formulation for activation of IL-6 production by APC, In general, an increasing proportion of QH-A vs QH-C increases the capacity to activate APC, The results demonstrate that the incorporated antigen and the adjuvant component in the same particle have the synergistic effects on immunogenicity. (C) 1997 Academic Press Limited.