CANINE HEMORRHAGIC ENTERITIS - DETECTION OF VIRAL PARTICLES BY ELECTRON-MICROSCOPY
CANINE HEMORRHAGIC ENTERITIS - DETECTION OF VIRAL PARTICLES BY ELECTRON-MICROSCOPY
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DOI：
10.1007/bf01320586
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发表时间：
1979-01-01
影响因子：
2.7
通讯作者：
PASTORET, PP
中科院分区：
文献类型：
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作者：
BURTONBOY, G;COIGNOUL, F;PASTORET, PP
在尸检中，几只死亡的狗表现出出血性腹泻的症状，在十二指肠和小肠上部有明显的粘膜损伤。病变粘膜的超薄切片研究显示在改变的肠隐窝细胞核中有类似细小病毒的颗粒。小肠内容物电镜阴性染色显示有许多直径为24 nm的病毒颗粒，其轮廓与二十面体形状一致。这些病毒粒子以1.43 g/cm3 CsCl的密度漂浮，并以4度凝集恒河猴和猪的红细胞。C.讨论了可能的病因作用。该病毒与犬的微小病毒和猫的泛白细胞减少病毒进行了比较。
At necropsy, several dogs which died showing symptoms of hemorrhagic diarrhea, had significant lesions of the mucosa in the duodenum and upper part of the small bowel. Study of ultrathin sections from the diseased mucosa revealed particles resembling parvoviruses in altered nuclei of cells of the intestinal crypts. EM examination of intestinal contents by negative staining showed the presence of many viral particles which have a diameter of 24 nm and whose profile is consistent with an icosahedral shape. These virions float at a density of 1.43 g/cm3 CsCl and agglutinate rhesus monkey and swine red blood cells at 4.degree. C. A possible etiological role is discussed. This virus is compared with the minute virus of canines and the feline panleukopenia virus.