Increased Salivary microRNAs That Regulate DJ-1 Gene Expression as Potential Markers for Parkinson's Disease
Increased Salivary microRNAs That Regulate DJ-1 Gene Expression as Potential Markers for Parkinson's Disease
复制标题
调节 DJ-1 基因表达的唾液 microRNA 增加，作为帕金森病的潜在标志物
DOI：
10.3389/fnagi.2020.00210
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发表时间：
2020-07-07
影响因子：
4.8
通讯作者：
Ye, Qinyong
中科院分区：
文献类型：
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作者：
Chen, Yanmei;Zheng, Jingxue;Ye, Qinyong
小分子RNA（small molecule RNAs，microRNA）是一类内源性、稳定的非编码RNA分子，可在转录后水平调控DJ-1等靶基因的表达。本研究旨在检测唾液microRNA的表达，并探讨其作为帕金森病（PD）唾液生物标志物的价值。通过病例对照研究，采用RT-qPCR技术检测30例PD患者和30例健康志愿者唾液中miR-874和miR-145 - 3p的表达。比较PD组和对照组唾液miR-874和miR-145 - 3p表达水平的差异，分析其与年龄、性别、病情、病程的相关性。结果发现，PD组和对照组唾液中miR-874和miR-145 - 3p均呈阳性表达，且PD组的表达高于对照组。唾液中miRNA-874和miR-145 - 3p的表达与患者年龄、性别、唾液中总RNA浓度、MMSE、MoCA、Hohn-Yahr分期、病程、嗅觉测试量表、MRSII、MRSIII评分无明显相关性。综上所述，在PD组和阳性表达的对照组中，PD组miR-874和miR-145 - 3p的表达水平均高于对照组。检测唾液中miR-874和miR-145 - 3p的表达可作为PD的辅助生物标志物。
Small molecule RNAs (microRNAs) are a kind of endogenous, stable, and noncoding RNA molecule that can regulate the expression of target genes such as DJ-1 at the posttranscriptional level. This study aimed to detect the expression of salivary microRNAs and to discover their value as a salivary potential biomarker for Parkinson's disease (PD). Through a case-control study, RT-qPCR technology was used to detect the expression of miR-874 and miR-145-3p in the saliva of 30 PD patients and 30 healthy volunteers. Then we compared the differences in the expression levels of salivary miR-874 and miR-145-3p between the PD group and the control group and analyzed the correlation between the expression of salivary miR-874 and miR-145-3p in terms of age, gender, disease condition, and disease course. We found that salivary miR-874 and miR-145-3p were both positively expressed in the PD group and control group, and their expression in the PD group was higher than that in the control group. The expression of salivary miRNA-874 and miR-145-3p had no clear correlation to age, gender, total RNA concentrations in saliva, the score of UPDRSII, UPDRSIII, olfactory test scale, MMSE, MoCA, Hohn-Yahr stage and disease course. In conclusion, in the PD group and the control group with positive expression, the expression levels of miR-874 and miR-145-3p in the PD group were higher than those in the control group. The detection of miR-874 and miR-145-3p expression in saliva can be used as an auxiliary biomarker for PD.