Short-term daily exercise activity enhances endothelial NO synthesis in skeletal muscle arterioles of rats.
Short-term daily exercise activity enhances endothelial NO synthesis in skeletal muscle arterioles of rats.
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短期日常运动活动增强大鼠骨骼肌小动脉内皮NO的合成。
DOI：
10.1152/jappl.1994.76.5.2241
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Kaley,G
中科院分区：
文献类型：
--
作者：
Sun,D;Huang,A;Koller,A;Kaley,G
我们的目的是测试的假设，作为短期的日常运动的结果，内皮细胞的控制小动脉平滑肌被改变。大鼠在跑步机上跑步，每天一次，5天/周，持续2-4周（逐渐增加强度，在第3周开始时以22 m/min的速度以1%的等级跑26分钟，在第4周开始时以28 m/min的速度以2%的等级跑38分钟），而对照组保持久坐（SED）。大鼠股薄肌的小动脉插管和加压自发肌源性张力，这是轻微增强运动（EX）相比，SED大鼠小动脉。在80 mmHg压力下，SED和EX大鼠小动脉的被动（无Ca（2+）溶液）和主动直径分别为105.4 +/- 3.8和55.1 +/- 2.3微米以及107.1 +/- 3.4和50.2 +/- 2.2微米。EX小动脉对硝普钠（10（-8）-10（-6）M）的剂量依赖性扩张和对去甲肾上腺素（10（-8）-10（-6）M）的收缩不受影响，而对腺苷（10（-6）-10（-4）M）的扩张显著减少。相反，与SED大鼠相比，EX大鼠的小动脉对乙酰胆碱（ACh; 5 × 10（-9）-10（-7）M）和L-精氨酸[一氧化氮（NO）的前体; 10（-4）-10（-3）M]的剂量依赖性扩张显著增强（分别增加33- 78%和57- 75%）。SED组和EX组小动脉对亚硝酸钠的反应没有差异。（250字处删节）
We aimed to test the hypothesis that as a consequence of short-term daily bouts of exercise the control of arteriolar smooth muscle by endothelium is altered. Rats ran on a treadmill once a day, 5 days/wk, for 2–4 wk (with gradually increasing intensity, up to 26 min at 22 m/min at a 1% grade by the beginning of the 3rd wk and up to 38 min at 28 m/min at a 2% grade by the beginning of the 4th wk) while a control group remained sedentary (SED). Cannulated and pressurized arterioles of rat gracilis muscle developed spontaneous myogenic tone, which was slightly enhanced in exercised (EX) compared with SED rat arterioles. At 80 mmHg pressure, the passive (Ca(2+)-free solution) and active diameters of SED and EX rat arterioles were 105.4 +/- 3.8 and 55.1 +/- 2.3 microns and 107.1 +/- 3.4 and 50.2 +/- 2.2 microns, respectively. Dose-dependent dilations to sodium nitroprusside (10(-8)-10(-6) M) and constrictions to norepinephrine (10(-8)-10(-6) M) were not affected in EX arterioles, whereas dilations to adenosine (10(-6)-10(-4) M) were significantly reduced. In contrast, dose-dependent dilations to acetylcholine (ACh; 5 x 10(-9)-10(-7) M) and L-arginine [precursor of nitric oxide (NO); 10(-4)-10(-3) M] were significantly enhanced (by 33–78 and 57–75%, respectively) in arterioles of EX compared with those of SED rats. Responses of arterioles to sodium nitrite were not different in SED and EX groups.(ABSTRACT TRUNCATED AT 250 WORDS)