DETECTION BY POLYMERASE CHAIN-REACTION OF GENES ENCODING AMINOGLYCOSIDE-MODIFYING ENZYMES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS ISOLATES OF EPIDEMIC PHAGE TYPES
DETECTION BY POLYMERASE CHAIN-REACTION OF GENES ENCODING AMINOGLYCOSIDE-MODIFYING ENZYMES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS ISOLATES OF EPIDEMIC PHAGE TYPES
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DOI：
10.1099/00222615-41-4-282
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发表时间：
1994-10-01
影响因子：
3
通讯作者：
HANNECARTPOKORNI, E
中科院分区：
文献类型：
--
作者：
VANHOOF, R;GODARD, C;HANNECARTPOKORNI, E
应用聚合酶链反应（PCR）技术对氨基糖苷类和甲氧西林耐药金黄色葡萄球菌流行株中氨基糖苷类修饰酶AAC（6 '）-APH（2''）、APH（3'）III和ANT（4'4''）的aacA-aphD、aphA 3和aadC基因以及甲氧西林耐药决定簇mecA进行了鉴定。在1980年至1985年期间收集的37株和81株从1991年至1992年期间从10个不同的比利时医院获得。早期的流行性分离株的特征是噬菌体组III的噬菌体C型（6/47/54/75），而组III的另外两种流行性噬菌体类型-A型（77）和B型（47/54/75/77/84/85）-在第二时期的分离株中最常见。双功能AAC（6 '）-APH（2“）是最常见的酶。1991-1992年期间APH（3 '）III的流行率显著下降，而ANT（4'，4“）仅在这一时期的分离株中发现。1991-1992年分离株的耐药机制较为复杂，所有分离株均检测到mecA基因。PCR结果与放射化学磷酸纤维素纸结合试验结果一致。从1991-1992年期间的分离株表现出显着更高水平的乙酰转移酶活性比20世纪80年代的分离株。
The polymerase chain reaction (PCR) was used to identify the aacA-aphD, aphA3 and aadC genes, encoding the aminoglycoside-modifying enzymes AAC(6')-APH(2''), APH(3')III and ANT(4' 4''), respectively, and the methicillin resistance determinant mecA, in epidemic aminoglycoside and methicillin-resistant isolates of Staphylococcus aureus. In total, 37 isolates collected in the period 1980-1985 and 81 isolates from the period 1991-1992 were obtained from 10 different Belgian hospitals. Epidemic isolates from the earlier period were characterised by phage type C (6/47/54/75) of phage group III, whereas two other epidemic phage types of group III-types A (77) and B (47/54/75/77/84/85)-were commonest in isolates from the second period. The bifunctional AAC(6')-APH(2'') was the enzyme encountered most frequently. The prevalence of APH(3')III decreased significantly in the 1991-1992 period, while ANT(4', 4'') was found solely in isolates from this period. Resistance mechanisms were more complex in isolates from the 1991-1992 period and the mecA gene was detected in all isolates. The PCR results corresponded well with those obtained in the radiochemical phosphocellulose paper binding assay. Isolates from the 1991-1992 period were shown to express significantly higher levels of acetyltransferase activity than isolates from the 1980s.