In Vivo Study of Biodistribution and Urinary Excretion of Surface-Modified Silica Nanoparticles
In Vivo Study of Biodistribution and Urinary Excretion of Surface-Modified Silica Nanoparticles
复制标题
表面修饰二氧化硅纳米粒子的生物分布和尿液排泄的体内研究
DOI：
10.1021/ac801882g
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发表时间：
2008-12-15
影响因子：
7.4
通讯作者：
Zhang, Pengfei
中科院分区：
文献类型：
--
作者：
He, Xiaoxiao;Nie, Hailong;Zhang, Pengfei
利用体内光学成像系统原位研究了不同表面修饰的二氧化硅纳米粒子（SiNPs）在小鼠体内的生物分布和尿排泄情况。制备了三种表面修饰的SiNPs，包括羟基修饰的SiNPs（OH - SiNPs）、羧基修饰的SiNPs（COOH - SiNPs）和聚乙二醇修饰的SiNPs（PEG - SiNPs），其尺寸均约为45 nm，并掺杂了RuBPY用于成像。通过静脉注射这些SiNPs，然后使用Maestro体内成像系统进行体内荧光追踪，结果表明OH - SiNPs、COOH - SiNPs和PEG - SiNPs都能从全身血液循环中清除，但清除时间和随后在生物器官中的沉积都取决于SiNPs的表面化学修饰。例如，PEG - SiNPs比OH - SiNPs和COOH - SiNPs表现出相对较长的血液循环时间以及较低的网状内皮系统器官摄取率。更有趣的是，膀胱体内实时成像的主要信号以及尿液排泄研究表明，所有三种静脉注射的尺寸约为45 nm的SiNPs都部分通过肾脏排泄途径排出。通过对器官进行体外光学成像、对尿液样本进行透射电子显微镜成像以及能量色散X射线光谱分析，进一步证实了这些结论。这些发现对于将SiNPs用作活体动物的递送系统和成像工具具有直接影响。此外，我们的结果表明，体内光学成像方法有助于通过使用掺杂在二氧化硅基质中的发光染料作为同步信号来体内感知SiNPs的生物学效应。
The biodistribution and urinary excretion of different surface-modified silica nanoparticles (SiNPs) in mice were investigated in situ using an in vivo optical imaging system. Three types of surface-modified SiNPs, including OH-SiNPs, COOH-SiNPs, and PEG-SiNPs with a size of similar to 45 nm, have been prepared with RuBPY doped for imaging purposes. Intravenous (iv) injection of these SiNPs followed by fluorescence tracing in vivo using the Maestro in vivo imaging system indicated that OH-SiNPs, COOH-SiNPs, and PEG-SiNPs were all cleared from the systemic blood circulation, but that both the clearance time and subsequent biological organ deposition were dependent on the surface chemical modification of the SiNPs. Thus, for instance, the PEG-SiNPs exhibited relatively longer blood circulation times and lower uptake by the reticuloendothelial system organs than OH-SiNPs and COOH-SiNPs. More interestingly, in vivo real-time imaged dominant signal in bladder and urine excretion studies revealed that all three types of iv-injected SiNPs with a size of similar to 45 nm were partly excreted through the renal excretion route. These conclusions were further confirmed through ex vivo organ optical imaging and TEM imaging and energy-dispersed X-ray spectrum analysis of urine samples. These findings would have direct implications for the use of SiNPs as delivery systems and imaging tools in live animals. Furthermore, our results demonstrate that the in vivo optical imaging method is helpful for in vivo sensing the biological effects of SiNPs by using luminescent dye doped in the silica matrix as a synchronous signal.