Novel outwardly rectifying anion conductance in Xenopus oocytes.
Novel outwardly rectifying anion conductance in Xenopus oocytes.
复制标题
非洲爪蟾卵母细胞中新型外向整流阴离子电导。
DOI：
10.1007/s00424-004-1324-z
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Arreola,Jorge
中科院分区：
文献类型：
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作者：
Reyes,JuanP;Hernandez-Carballo,CarmenY;Pérez-Cornejo,Patricia;Meza,Ulises;Espinosa-Tanguma,Ricardo;Arreola,Jorge
We describe a novel, strongly outwardly rectifying anion current inXenopus laevisoocytes, that we have namedICl,Or. The properties ofICl,Orare different from those of any other anion conductance previously described in these cells. Typically,ICl,Oramplitude was small when extracellular Cl−(Cle) was the permeant anion. However, when Clewas replaced by lyotropic anionsICl,Orbecame evident as a time-independent current.ICl,Orwas voltage dependent and showed a remarkable outwards rectification with little or no inwards tail current. The relative selectivity sequence determined from current amplitudes was: SCN−≥ClO4−>I−>Br−≥NO3−>Cl−.ICl,Orwas insensitive to Gd3+but was blocked by micromolar concentrations of niflumic acid, DIDS or Zn2+. Furthermore,ICl,Orwas not affected by buffering intracellular Ca2+with BAPTA. Low extracellular pH inhibitedICl,Orwith a pK of 5.8. We propose thatICl,Ormight result from activation of endogenous ClC-5-like Cl−channels present inXenopusoocytes.
