Comparison between Candida albicans agglutinin-like sequence gene expression patterns in human clinical specimens and models of vaginal candidiasis
Comparison between Candida albicans agglutinin-like sequence gene expression patterns in human clinical specimens and models of vaginal candidiasis
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DOI：
10.1128/iai.73.3.1656-1663.2005
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发表时间：
2005-03-01
影响因子：
3.1
通讯作者：
Hoyer, LL
中科院分区：
文献类型：
--
作者：
Cheng, G;Wozniak, K;Hoyer, LL
应用逆转录-PCR技术对临床阴道分泌物标本和阴道念珠菌病模型系统中的白念珠菌凝集素样序列（ALS）家族中8个基因的表达进行了研究。尽管在所有的临床标本中均检测到了所有ALS基因的表达，但ALS 1、ALS 2、ALS 3和ALS 9转录本的检测频率最高，而ALS 4和ALS 5的表达检测频率最低。实验室菌株3153 A和两株C.使用两种阴道念珠菌病模型研究从临床样本中分离的白色念珠菌菌株，以确定这些模型在基因表达水平上与临床样本的相似程度。小鼠阴道炎模型中的ALS基因表达模式与临床标本中的相同。感染后7天收集的标本中检测到更多的ALS基因的表达相比，在4天收集的。当三种C.在重建的人阴道上皮模型中测试白色念珠菌菌株。在该模型中，最不频繁检测到ALS 4、ALS 5、ALS 6和ALS 7的表达。在早期时间点观察到ALS 4表达的阴性或减弱的信号，表明在接种细胞中强的ALS 4表达在接触C.白色念珠菌与阴道上皮细胞。这里提供的数据支持的结论，宿主位点特异性的影响AILS基因的表达和验证使用的实验模型评估als/als突变株的表型。
Expression of the eight genes in the Candida albicans agglutinin-like sequence (ALS) family was studied by reverse transcription-PCR of RNA isolated from clinical vaginal fluid specimens and vaginal candidiasis model systems. Although expression of all ALS genes was detected across the set or clinical specimens, ALS1, ALS2, ALS3, and ALS9 transcripts were detected most frequently, and expression of ALS4 and ALS5 was detected least frequently. Laboratory strain 3153A and two C. albicans strains isolated from the clinical specimens were studied using two models of vaginal candidiasis to determine how closely these models mimicked the clinical specimens at the level of gene expression. ALS gene expression patterns in a murine vaginitis model were identical to those from the clinical specimens. Expression of more ALS genes was detected in specimens collected 7 days after infection compared to those collected at 4 days. Similar patterns of ALS gene expression were observed when the three C. albicans strains were tested in the reconstituted human vaginal epithelium model. In this model, expression of ALS4, ALS5, ALS6, and ALS7 was least frequently detected. Negative or weakened signals for ALS4 expression were observed at early time points, suggesting that ALS4 expression, which was strong in the inoculum cells, was down-regulated upon contact of C. albicans with vaginal epithelial cells in this model. The data presented here support the conclusion of host-site-specific influences on AILS gene expression and validate the use of the experimental models for evaluating the phenotype of als/als mutant strains.