Spatio-angular fluorescence microscopy II. Paraxial 4f imaging
Spatio-angular fluorescence microscopy II. Paraxial 4f imaging
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DOI：
10.1364/josaa.36.001346
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发表时间：
2019-08-01
影响因子：
1.9
通讯作者：
La Riviere, Patrick
中科院分区：
文献类型：
--
作者：
Chandler, Talon;Shroff, Hari;La Riviere, Patrick
我们调查的单视图荧光显微镜在4f几何成像荧光偶极子时，不使用标量或标量近似的属性。我们表明，这种成像系统具有空间角度的频带限制，我们利用频带限制进行有效的模拟。值得注意的是，我们表明，近轴荧光显微镜记录的信息在焦点荧光团的合奏的面外取向。此外，我们表明，ESTA近似可能会导致有偏见的荧光团浓度的估计，但这些偏差是小的，当样品中包含许多随机取向的荧光团在每个可分辨的体积或不受约束的旋转荧光团。(C)2019美国光学学会
We investigate the properties of a single-view fluorescence microscope in a 4f geometry when imaging fluorescent dipoles without using the monopole or scalar approximations. We show that this imaging system has a spatio-angular band limit, and we exploit the band limit to perform efficient simulations. Notably, we show that information about the out-of-plane orientation of ensembles of in-focus fluorophores is recorded by paraxial fluorescence microscopes. Additionally, we show that the monopole approximation may cause biased estimates of fluorophore concentrations, but these biases are small when the sample contains either many randomly oriented fluorophores in each resolvable volume or unconstrained rotating fluorophores. (C) 2019 Optical Society of America