Analysis of expressed sequence tags from biodiesel plant Jatropha curcas embryos at different developmental stages
Analysis of expressed sequence tags from biodiesel plant Jatropha curcas embryos at different developmental stages
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DOI：
10.1016/j.plantsci.2011.08.003
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发表时间：
2011-12-01
期刊：
影响因子：
5.2
通讯作者：
Xu, Zeng-Fu
中科院分区：
文献类型：
--
作者：
Chen, Mao-Sheng;Wang, Gui-Juan;Xu, Zeng-Fu
麻风树被认为是一种潜在的生物柴油原料植物，其种子含油量高达 40%。然而，目前人们对麻风树的种子生物学知之甚少。因此，了解麻风树种子的发育和脂质代谢机制是有价值的。在本研究中，利用种子发育不同阶段的麻风树胚胎的 mRNA 构建了三个 cDNA 文库。从这些文库中总共产生了 9844 个表达序列标签 (EST)，从中获得了 1070 个重叠群和 3595 个单例。在麻风树胚的三个cDNA文库中发现了107个unigenes的差异表达，表明这些基因可能在种子发育中发挥关键作用。我们分别鉴定了 59 个和 61 个可能参与麻风树种子发育和脂质代谢的单基因。其中一些基因也可能在植物的胚胎发生、形态发生、防御反应和适应机制中发挥重要作用。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
Jatropha curcas is considered a potential biodiesel feedstock plant whose seeds contain up to 40% oil. However, little is currently known about the seed biology of Jatropha. Therefore, it would be valuable to understand the mechanisms of development and lipid metabolism in Jatropha seeds. In the present study, three cDNA libraries were constructed with mRNA from Jatropha embryos at different stages of seed development. A total of 9844 expressed sequence tags (ESTs) were produced from these libraries, from which 1070 contigs and 3595 singletons were obtained. One hundred and seven unigenes were found to be differentially expressed in the three cDNA libraries of Jatropha embryos, indicating that these genes may play key roles in seed development. We have identified 59 and 61 unigenes that might be involved in the development and lipid metabolism in Jatropha seeds, respectively. Some of these genes may also play important roles in embryogenesis, morphogenesis, defense response and adaptive mechanisms in plants. (C) 2011 Elsevier Ireland Ltd. All rights reserved.