IFN-γ plays an essential role in the pathogenesis of gastric lymphoid follicles formation caused by Helicobacter suis infection
IFN-γ plays an essential role in the pathogenesis of gastric lymphoid follicles formation caused by Helicobacter suis infection
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DOI：
10.1111/j.1574-695x.2011.00823.x
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发表时间：
2011-10-01
影响因子：
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通讯作者：
Azuma, Takeshi
中科院分区：
文献类型：
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作者：
Mimura, Takuya;Yoshida, Masaru;Azuma, Takeshi
猪。在这项研究中，我们的目的是评估辅助性T细胞在猪螺杆菌感染诱导的胃淋巴滤泡发育中的作用。C57 BL/6 J小鼠经口接种H.猪。感染后6周，苏木精-伊红染色可见胃粘膜内有胃淋巴滤泡，且在整个感染期内滤泡数量增多。免疫组化检查显示淋巴滤泡由B细胞、CD 4阳性辅助性T细胞和树突状细胞（DC）组成。结果还显示，在感染后12周，胃粘膜中干扰素-g（IFN-γ）的mRNA表达水平显著增加。IFN-γ缺陷小鼠感染H.感染后12周，虽然IL-4缺陷小鼠感染H. SUIS与在野生型小鼠中观察到的相似。结论：IFN-γ是一种Th 1型细胞因子，在H.猪感染，表明CD 4阳性T细胞和DC有助于胃淋巴滤泡的扩张。
suis. In this study, we aimed to assess the role of helper T cells in the development of gastric lymphoid follicles induced by Helicobacter suis infection. C57BL/6J mice were orally inoculated with H. suis. Six weeks after infection, gastric lymphoid follicles were observed in the gastric mucosa by hematoxylin and eosin staining, and the number of follicles was increased throughout the infection period. An immunohistological examination showed that the lymphoid follicles were composed of B cells, CD4-positive helper T cells, and dendritic cells (DC). It was also revealed that the mRNA expression level of interferon-g (IFN-gamma) in the gastric mucosa was significantly increased at 12 weeks after infection. No gastric lymphoid follicles were detected in IFN-gamma-deficient mice that had been infected with H. suis at 12 weeks after infection, although the development of lymphoid follicles in IL-4-deficient mice infected with H. suis was similar to that seen in the wild-type mice. In conclusion, IFN-gamma, a Th1 cytokine, is deeply involved in the pathogenesis of gastric lymphoid follicles induced by H. suis infection, and it is suggested that CD4-positive T cells and DC aid in the expansion of gastric lymphoid follicles.