Application of In Vivo Laser Scanning Confocal Microscopy for Evaluation of Ocular Surface Diseases: Lessons Learned From Pterygium, Meibomian Gland Disease, and Chemical Burns
Application of In Vivo Laser Scanning Confocal Microscopy for Evaluation of Ocular Surface Diseases: Lessons Learned From Pterygium, Meibomian Gland Disease, and Chemical Burns
复制标题
活体激光扫描共焦显微镜在眼表疾病评估中的应用：翼状胬肉、睑板腺疾病和化学烧伤的经验教训
DOI：
10.1097/ico.0b013e318227fcd9
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发表时间：
2011-10-01
期刊：
影响因子：
2.8
通讯作者：
Sun, Xinghuai
中科院分区：
文献类型：
--
作者：
Wang, Yan;Le, Qihua;Sun, Xinghuai
活体激光扫描共聚焦显微镜（LSCM）已被广泛用于在活体眼中从细胞水平评估眼表疾病所导致的变化。在这篇综述中，我们重点关注其在翼状胬肉、睑板腺（MG）疾病和化学烧伤诊断中的应用。翼状胬肉发生的组织病理学变化可使用活体LSCM原位检查。可以观察到在翼状胬肉和角膜交界处的变化，而这些变化在切除的组织样本中是无法观察到的。睑板腺在维持眼表健康方面起着重要作用。睑板腺功能障碍（MGD）是最常见的眼表疾病之一。活体LSCM的使用有助于MGD的诊断，并提供了一种检查睑板腺腺泡单位微观结构并测量其大小的方法。活体LSCM也为理解睑板腺对眼表健康的作用提供了新的视角。化学烧伤是最常见的眼部损伤之一，活体LSCM可以提供角膜表面杯状细胞的图像，这是角膜缘干细胞缺乏的一个标志。活体LSCM在评估化学烧伤方面的应用需要拓展，以便能够评估角膜缘结构以及眼部重建手术后眼表的变化。
In vivo laser scanning confocal microscopy (LSCM) has been widely used to evaluate the alterations caused by ocular surface diseases at a cellular level in the living eye. In this review, we focus on its use in the diagnosis of pterygium, meibomian gland (MG) disease, and chemical burns. Histopathologic changes occurring in pterygium can be examined in situ using in vivo LSCM. Alterations at the junction of the pterygium and the cornea, which cannot be observed in excised tissue samples, can be observed. MGs play an important role in maintaining the health of the ocular surface. Meibomian gland dysfunction (MGD) is one of the most common ocular surface diseases. The use of in vivo LSCM helps in the diagnosis of MGD and provides a way to examine the microstructure of MG acinar units and measure their size. In vivo LSCM also provides a new perspective in understanding the contribution of the MG to the health of the ocular surface. Chemical burns are one of the most common ocular injuries, and in vivo LSCM can provide images of the goblet cells on the corneal surface. This is a hallmark of limbal stem cell deficiency. The application of in vivo LSCM to assessing chemical burns requires extension, allowing for evaluation of the limbus structure and ocular surface changes after reconstructive ocular surgery.