Salt effect on yield and composition of shoot essential oil and trichome morphology and density on leaves of Mentha pulegium
Salt effect on yield and composition of shoot essential oil and trichome morphology and density on leaves of Mentha pulegium
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DOI：
10.1016/j.indcrop.2009.06.003
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发表时间：
2009-11-01
影响因子：
5.9
通讯作者：
Smaoui, Abderrazak
中科院分区：
文献类型：
--
作者：
Karray-Bouraoui, Najoua;Rabhi, Mokded;Smaoui, Abderrazak
研究了盐胁迫对薄荷地上部挥发油产量、组成的影响，以及影响其生物合成的结构。采用水蒸气蒸馏法提取竹笋中的环氧乙烷，用GC-MS法测定其组成。取顶叶和基叶进行显微镜分析：用环境扫描电子显微镜（ESEM）和体视显微镜（SM）直接观察小的新鲜样品，而不进行固定或金属化。新鲜分离的表皮用于光学显微镜（LM）。盐胁迫使EO产量提高了约2.75倍，并影响了主要化合物薄荷酮的百分比（约为51%），增加了胡薄荷酮的百分比。发现构成单萜类的乌藨子酮、胡薄荷酮和新薄荷酮是主要成分。解剖学研究显示了三种类型的腺毛：（i）非腺毛，多细胞，单毛;（ii）小，头状腺毛;（iii）盾状腺毛。在对照植物中，毛状体的密度和大小随叶侧（远轴或近轴）和发育阶段而变化。盐胁迫导致影响两侧毛状体分布和大小的显着修改。(C)2009爱思唯尔有限公司版权所有。
The aim of the present work was to study salt effect on the yield and composition of shoot essential oil (EO) and the structures responsible for its biosynthesis in Mentha pulegium L. Shoot EO was extracted by hydrodistillation and composition was determined by GC-MS method. Apical and basal leaves were taken for microscopy analyses: small fresh samples were observed directly without fixation or metallisation with environmental scanning electron microscope (ESEM) and stereomicroscope (SM). Fresh separate epidermis was used for light microscopy (LM). Salt stress enhanced EO yield by about 2.75 times and affected the percentage of menthone, which is the major compound (similar to 51%), increasing that of pulegone. Menthone, pulegone, and neomenthol constituting the monoterpene class were found to be the principal components. The anatomical study showed three types of trichomes: (i) non-glandular, multicellular, simple hairs; (ii) small, capitate glandular trichomes; (iii) and peltate glandular trichomes. In control plants, the density and size of trichomes varied with leaf side (abaxial or adaxial) and developmental stage. Salt stress results in significant modifications affecting trichome distribution and size on both sides. (C) 2009 Elsevier B.V. All rights reserved.