Changes in the Phosphoproteome and Metabolome Link Early Signaling Events to Rearrangement of Photosynthesis and Central Metabolism in Salinity and Oxidative Stress Response in Arabidopsis
Changes in the Phosphoproteome and Metabolome Link Early Signaling Events to Rearrangement of Photosynthesis and Central Metabolism in Salinity and Oxidative Stress Response in Arabidopsis
复制标题
拟南芥磷酸化蛋白质组和代谢组的变化将早期信号事件与盐度和氧化应激反应中光合作用和中枢代谢的重排联系起来
DOI：
10.1104/pp.15.01486
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发表时间：
2015-12-01
期刊：
影响因子：
7.4
通讯作者：
Hoehenwarter, Wolfgang
中科院分区：
文献类型：
--
作者：
Chen, Yanmei;Hoehenwarter, Wolfgang
盐胁迫和氧化胁迫是影响和限制农作物产量的主要因素。控制植物对非生物胁迫反应的分子和生物化学过程常常以还原论的方式进行研究。在这里，我们报告了一个系统的方法相结合的代谢标记和磷酸蛋白质组学捕捉早期信号事件与定量代谢组学分析和酶活性测定，以确定盐和氧化胁迫对植物生理的影响。K+和Na+转运蛋白磷酸化模式的协调变化，表明动态离子稳态的适应盐胁迫的重要性。独特的磷酸化位点被发现为拟南芥（拟南芥）SNF 1激酶同源10和11，表明其在压力调节反应的中心作用。7蔗糖非发酵1-相关蛋白激酶2激酶显示不同水平的磷酸化在多个丝氨酸/苏氨酸残基在其激酶结构域的压力后，显示时间上不同的调制的各种亚型。盐度和氧化应激也导致光合作用的核心蛋白质的蛋白质磷酸化的变化，特别是状态转换和捕光II复合蛋白所需的激酶状态转换蛋白7。此外，还观察到应激诱导的中枢代谢酶磷酸化的变化。这些蛋白质的磷酸化模式与酶活性的变化同时发生。这反映在代谢物水平的改变上，如蔗糖和果糖、糖酵解中间体和氨基酸。总之，我们的研究为盐和氧化应激反应中的早期信号传导之间的联系提供了证据，该信号传导调节光合作用的状态转换和初级代谢的重排。
Salinity and oxidative stress are major factors affecting and limiting the productivity of agricultural crops. The molecular and biochemical processes governing the plant response to abiotic stress have often been researched in a reductionist manner. Here, we report a systemic approach combining metabolic labeling and phosphoproteomics to capture early signaling events with quantitative metabolome analysis and enzyme activity assays to determine the effects of salt and oxidative stress on plant physiology. K+ and Na+ transporters showed coordinated changes in their phosphorylation pattern, indicating the importance of dynamic ion homeostasis for adaptation to salt stress. Unique phosphorylation sites were found for Arabidopsis (Arabidopsis thaliana) SNF1 kinase homolog10 and 11, indicating their central roles in the stress-regulated responses. Seven Sucrose Nonfermenting1-Related Protein Kinase2 kinases showed varying levels of phosphorylation at multiple serine/threonine residues in their kinase domain upon stress, showing temporally distinct modulation of the various isoforms. Salinity and oxidative stress also lead to changes in protein phosphorylation of proteins central to photosynthesis, in particular the kinase State Transition Protein7 required for state transition and light-harvesting II complex proteins. Furthermore, stress-induced changes of the phosphorylation of enzymes of central metabolism were observed. The phosphorylation patterns of these proteins were concurrent with changes in enzyme activity. This was reflected by altered levels of metabolites, such as the sugars sucrose and fructose, glycolysis intermediates, and amino acids. Together, our study provides evidence for a link between early signaling in the salt and oxidative stress response that regulates the state transition of photosynthesis and the rearrangement of primary metabolism.