TUFTSIN-MACROPHAGE INTERACTION - SPECIFIC BINDING AND AUGMENTATION OF PHAGOCYTOSIS
TUFTSIN-MACROPHAGE INTERACTION - SPECIFIC BINDING AND AUGMENTATION OF PHAGOCYTOSIS
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DOI：
10.1002/jcp.1041000106
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发表时间：
1979-01-01
影响因子：
5.6
通讯作者：
FRIDKIN, M
中科院分区：
文献类型：
--
作者：
BARSHAVIT, Z;GOLDMAN, R;FRIDKIN, M
在22℃下研究了[3H]促吞噬素与正常和体内刺激的小鼠腹膜巨噬细胞群的结合。 C.[ 3 H]促吞噬素与巯基乙酸盐刺激的巨噬细胞的结合是快速且可饱和的，平衡Kd（从斯卡查德图计算）为5.3×。 10-8 M。计算出每个巨噬细胞的结合位点数量约为 72,000 个。与未标记的促吞噬素的结合竞争研究产生了 5.0 倍的 Kd。 10-8 M. [3H] [N-乙酰基-Thr1] tuftsin 是一种 tuftsin 的无活性类似物，无法与硫代乙醇酸刺激的巨噬细胞特异性结合。 [N-乙酰-Thr1]tuftsin 和三肽[Des-Arg4]tuftsin 未能竞争tuftsin 结合位点，而[D-Arg4]tuftsin 是一种具有小tuftsin 样活性的类似物，对[3H]tuftsin 结合表现出较低程度的抑制。因此，四肽的结合涉及相当高程度的特异性。正常巨噬细胞群以及由硫代乙醇酸盐、刀豆球蛋白 A、淀粉、矿物油、葡聚糖和卡介苗刺激诱导的 6 种不同巨噬细胞群，表现出相似程度的 [3H]tuftsin 结合。小棒状杆菌 [痤疮丙酸杆菌] (CP) 刺激的巨噬细胞显示出 6 至 10 倍的 Tuftsin 结合能力。在类似的实验条件下，小鼠成纤维细胞和淋巴细胞制剂显示没有可检测到的特异性结合。 Tuftsin 增强了正常和刺激巨噬细胞的吞噬反应，评估了通过 Fc 受体和非特异性受体介导的吞噬作用。 CP刺激的巨噬细胞在用tuftsin处理后并没有表现出增加的吞噬反应。
The binding of [3H]tuftsin to normal and in vivo stimulated mouse peritoneal macrophage populations was studied at 22.degree. C. The [3H]tuftsin binding to thioglycollate-stimulated macrophages was rapid and saturable, with an equilibrium Kd (calculated from a Scatchard plot) of 5.3 .times. 10-8 M. The calculated number of binding sites per macrophage amounts to approximately 72,000. Binding competition studies with unlabeled tuftsin yielded a Kd of 5.0 .times. 10-8 M. [3H] [N-Acetyl-Thr1] tuftsin, an inactive analog of tuftsin, failed to bind specifically to thioglycollate-stimulated macrophages. [N-Acetyl-Thr1]tuftsin and the tripeptide [Des-Arg4]tuftsin failed to compete for tuftsin binding sites, while [D-Arg4]tuftsin, an analog with small tuftsin-like activity, exhibited a low degree of inhibition of [3H]tuftsin binding. Thus a rather high degree of specificity is involved in the binding of the tetrapeptide. Normal as well as 6 different macrophage populations induced by stimulation with thioglycollate, concanavalin A, starch, mineral oil, glucan and BCG, exhibited a similar degree of binding of [3H]tuftsin. Corynebacterium parvum [Propionibacterium acnes] (CP)-stimulated macrophages showed a 6- to 10-fold-lower capacity for tuftsin binding. Under similar experimental conditions, mouse fibroblast and lymphocyte preparations revealed no detectable specific binding. Tuftsin augmented the phagocytic response of normal and stimulated macrophages assessed for phagocytosis mediated via the Fc-receptor and via non-specific receptors. CP-stimulated macrophages did not exhibit an increased phagocytic response upon treatment with tuftsin.