Molecular characterization of Botrytis ourmia-like virus, a mycovirus close to the plant pathogenic genus Ourmiavirus
Molecular characterization of Botrytis ourmia-like virus, a mycovirus close to the plant pathogenic genus Ourmiavirus
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DOI：
10.1016/j.virol.2015.11.027
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发表时间：
2016-02-01
期刊：
影响因子：
3.7
通讯作者：
Ayllon, Maria A.
中科院分区：
文献类型：
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作者：
Donaire, Livia;Rozas, Julio;Ayllon, Maria A.
利用Illumina平台，通过下一代测序技术获得了一种新的植物病原真菌葡萄孢属（Botrytis）田间葡萄藤分离株中的单链RNA病毒，并对其进行了分子鉴定。与NCBI数据库的序列比较表明，该病毒与Ourmiavirus属植物病原病毒的RNA依赖性RNA聚合酶（RdRps）具有很强的同源性，因此将该病毒命名为Botrytis ourmia-like virus（BOLV）。BOLV有一个2169个核苷酸的开放阅读框，编码722个氨基酸的蛋白质，具有植物RNA病毒RdRps的保守结构域，如最保守的GDD活性结构域。我们的分析表明，BOLV在遗传学上更接近真菌Narnavirus和植物Ourmiavirus，而不是Narnaviridae科的Mitovirus。因此，我们认为BOLV可能代表Narnaviridae真菌病毒和植物ourmiaviruses之间的联系。(C)2015 Elsevier Inc. All rights reserved.
The molecular characterization of a novel single-stranded RNA virus, obtained by next generation sequencing using Illumina platform, in a field grapevine isolate of the plant pathogenic fungus Botrytis, is reported in this work. The sequence comparison of this virus against the NCBI database showed a strong identity with RNA dependent RNA polymerases (RdRps) of plant pathogenic viruses belonging to the genus Ourmiavirus, therefore, this novel virus was named Botrytis ourmia-like virus (BOLV). BOLV has one open reading frame of 2169 nucleotides, which encodes a protein of 722 amino acids showing conserved domains of plant RNA viruses RdRps such as the most conserved GDD active domain. Our analyses showed that BOLV is phylogenetically closer to the fungal Narnavirus and the plant Ourmiavirus than to Mitovirus of the family Narnaviridae. Hence, we proposed that BOLV might represent the link between fungal viruses of the family Narnaviridae and the plant ourmiaviruses. (C) 2015 Elsevier Inc. All rights reserved.