Paenibacillus yonginensis DCY84T induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress
Paenibacillus yonginensis DCY84T induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress
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DOI：
10.1016/j.micres.2015.01.007
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发表时间：
2015-01-01
影响因子：
6.7
通讯作者：
Yang, Deok Chun
中科院分区：
文献类型：
--
作者：
Sukweenadhi, Johan;Kim, Yeon-Ju;Yang, Deok Chun
目前的农业生产方法，例如不适当地使用化肥和杀虫剂，造成了许多健康和环境问题。植物促生菌（PGPB）在农业上的应用在世界范围内日益增加，并且似乎也是未来的趋势。基于这些有益的植物-微生物相互作用，有可能开发用于农业生物技术的微生物接种剂。本研究从福建永仁森林土壤中分离到10株细菌，通过体外促植物生长性状筛选，如吲哚乙酸（IAA）产量、溶磷试验和铁载体产量试验，筛选出2株PGPB候选菌株。用永宁类芽孢杆菌（Paenibacillus yonginensis）DCY 84（T）和云南微球菌PGPB 7接种拟南芥植株。盐胁迫、干旱胁迫和重金属（铝）胁迫表明，接种永仁杨DCY 84（T）的植株比对照植株具有更强的抗性。AtRSA 1、AtVQ 9和AtWRKY 8被用作盐度响应基因。选择了AtERD 15、AtRAB 18和AtLT 178来检查A.拟南芥对干旱胁迫的响应使用AtAIP、AtALS 3和AtALMT 1检查铝的应力响应。qRT-PCR结果表明，永仁杨DCY 84（T）基因能够提高植物对盐、干旱和铝胁迫的耐受性。永仁拟青霉DCY 84（T）在体外亲和性试验和对X. oryzaepv.水稻菲律宾生理小种6号（PXO 99）。在从Suncheon收集的沿海土壤上生长的P. yonginensis DCY 84（T）Chuchung栽培稻种子也记录了较好的发芽率和生长参数。因此，永宁片锈菌DCY 84（T）可作为一个有前途的PGPB菌株用于作物改良。(C)2015爱思唯尔有限公司All rights reserved.
Current agricultural production methods, for example the improper use of chemical fertilizers and pesticides, create many health and environmental problems. Use of plant growth-promoting bacteria (PGPB) for agricultural benefits is increasing worldwide and also appears to be a trend for the future. There is possibility to develop microbial inoculants for use in agricultural biotechnology, based on these beneficial plant-microbe interactions. For this study, ten bacterial strains were isolated from Yongin forest soil for which in vitro plant-growth promoting trait screenings, such as indole acetic acid (IAA) production, a phosphate solubilization test, and a siderophore production test were used to select two PGPB candidates. Arabidopsis thaliana plants were inoculated with Paenibacillus yonginensis DCY84(T) and Micrococcus yunnanensis PGPB7. Salt stress, drought stress and heavy metal (aluminum) stress challenges indicated that P. yonginensis DCY84(T)-inoculated plants were more resistant than control plants. AtRSA1, AtVQ9 and AtWRKY8 were used as the salinity responsive genes. The AtERD15, AtRAB18, and AtLT178 were selected to check A. thaliana responses to drought stress. Aluminum stress response was checked using AtAIP, AtALS3 and AtALMT1. The qRT-PCR results indicated that P. yonginensis DCY84(T) can promote plant tolerance against salt, drought, and aluminum stress. P. yonginensis DCY84(T) also showed positive results during in vitro compatibility testing and virulence assay against X. oryzae pv. oryzae Philippine race 6 (PXO99). Better germination rates and growth parameters were also recorded for the P. yonginensis DCY84(T) Chuchung cultivar rice seed which was grown on coastal soil collected from Suncheon. Based on these results, P. yonginensis DCY84(T) can be used as a promising PGPB isolate for crop improvement. (C) 2015 Elsevier GmbH. All rights reserved.