Genetic structure of Quercus gilva Blume in Japan as revealed by chloroplast DNA sequences
Genetic structure of Quercus gilva Blume in Japan as revealed by chloroplast DNA sequences
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DOI：
10.1139/cjb-2015-0025
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发表时间：
2015-12-01
期刊：
影响因子：
1.1
通讯作者：
Ide, Yuji
中科院分区：
文献类型：
--
作者：
Sugiura, Nami;Tang, Dingqin;Ide, Yuji
黄栎（Quercus gilva）是一种万年青栎属，原产于日本、中国和台湾。由于长期以来人类的影响，该物种在日本多个地区面临灭绝的威胁。本研究的目的是确定Q的遗传结构。gilva的叶绿体DNA（cpDNA）序列分析。我们收集了来自日本25个种群的123个样本，来自中国1个种群的8个样本，来自台湾5个种群的46个样本。对177个样品中的每一个进行了约1815bp的cpDNA测序。检测到13种单倍型，在三个地理上分离的国家中没有跨区域的单倍型分布。居群间遗传差异较大（G（ST）= 0.824，G '（ST）= 0.937）。在日本共检测到6种单倍型（单倍型1、2、3、4a、4b和5），其中单倍型4a最常见，在20个居群中检测到，除单倍型4b外，其他罕见单倍型均出现在物种分布的边缘。此外，四个单倍型（单倍型1，2，3，和5）是非常不同的占主导地位的单倍型（单倍型4a），有超过四个的cpDNA突变，除了一个单核苷酸重复序列，这表明这些罕见的单倍型的人群应该是单独保存。
Quercus gilva (Blume) is an evergreen oak species that is native to Japan, China, and Taiwan. Because of a long history of human impact, this species is threatened with extinction in several regions of Japan. The objective of this study was to identify the genetic structure of Q. gilva based on chloroplast DNA (cpDNA) sequencing analysis. We collected 123 samples from 25 populations in Japan, 8 samples from 1 population in China, and 46 samples from 5 populations in Taiwan. Approximately 1815 bp of cpDNA was sequenced for each of the 177 samples. Thirteen haplotypes were detected, with no cross-region distribution of haplotypes among the three geographically separated countries. There were large genetic differences among populations (G(ST) = 0.824, G'(ST) = 0.937). Six haplotypes (haplotypes 1, 2, 3, 4a, 4b, and 5) were detected in Japan; haplotype 4a was the most common, detected from 20 populations, and the other rare haplotypes, except for haplotype 4b, occurred at the edge of the species' distribution. In addition, four haplotypes (haplotypes 1, 2, 3, and 5) were quite different from the predominant haplotype (haplotype 4a), with more than four cpDNA mutations except for a mononucleotide repeat, suggesting that populations with these rare haplotypes should be conserved separately.