Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo.
Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo.
复制标题
通过体内重组和选择从噬菌体文库中纯化基因组序列。
DOI：
10.1093/nar/11.8.2427
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发表时间：
1983
影响因子：
14.9
通讯作者：
Seed,B
中科院分区：
文献类型：
--
作者：
Seed,B
克隆的基因已经通过利用体内同源相互重组的方法从重组DNA噬菌体文库中纯化。在这种方法中，“探针”序列被插入到非常小的质粒载体中，并引入到重组熟练的细菌细胞中。基因组噬菌体文库在细胞上繁殖，携带与探针同源的序列的噬菌体通过相互重组获得质粒的整合拷贝。携带整合质粒的噬菌体可以通过在适当的选择性条件下生长而从缺乏质粒整合体的噬菌体的较大库中纯化。图像
Cloned genes have been purified from recombinant DNA bacteriophage libraries by a method exploiting homologous reciprocal recombination in vivo. In this method 'probe' sequences are inserted in a very small plasmid vector and introduced into recombination-proficient bacterial cells. Genomic bacteriophage libraries are propagated on the cells, and phage bearing sequences homologous to the probe acquire an integrated copy of the plasmid by reciprocal recombination. Phage bearing integrated plasmids can be purified from the larger pool of phage lacking plasmid integrates by growth under the appropriate selective conditions. Images