Tobacco mosaic virus infection induces severe morphological changes of the endoplasmic reticulum
Tobacco mosaic virus infection induces severe morphological changes of the endoplasmic reticulum
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DOI：
10.1073/pnas.95.19.11169
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发表时间：
1998-09-15
影响因子：
11.1
通讯作者：
Beachy, RN
中科院分区：
文献类型：
--
作者：
Reichel, C;Beachy, RN
烟草花叶病毒(TMV)运动蛋白(MP)通过修饰胞间连丝，促进病毒在相邻细胞之间的传播。以往的研究表明，细胞骨架和内膜系统参与了这种转运。我们研究了烟草花叶病毒(TMV)感染对内质网(ER)的影响。荧光显微镜被用来显示，在感染的早期，内质网经历了戏剧性的形态变化，包括管状内质网转变为大的聚集体，在感染的后期恢复为管状内质网。这些变化与MP的积累和降解平行。此外，含有MP与GFP融合的融合蛋白聚集在这些大的ER聚集体中或之上。在没有病毒感染的情况下，MP-GFP的表达导致产生相同外观形式和大小的荧光聚集体。从受感染的叶片中分离出的微粒体内含有MP。我们发现MP似乎表现为一个完整的内质网膜蛋白，并暴露在内质网的胞浆表面。讨论了MP与ER的关系的重要性及其在感染的细胞内和细胞间传播中的可能作用。
The tobacco mosaic virus (TMV) movement protein (MP) facilitates transport of virus infection between adjacent cells by modifying plasmodesmata. Previous studies suggested that the cytoskeleton and the endomembrane system are involved in this transport. We examined the effects of TMV infection on the endoplasmic reticulum (ER) in transgenic Nicotiana benthamiana that accumulate the green fluorescent protein (GFP) in the ER. Fluorescence microscopy was used to show that early in infection the ER undergoes dramatic morphological changes that include the conversion of tubular ER into large aggregates that revert to tubular ER in later stages of infection. These changes parallel MP accumulation and degradation. Furthermore, a fusion protein comprising MP fused to GFP accumulates in or on these large aggregates of ER. Expression of MP-GFP in the absence of virus infection led to the production of fluorescent aggregates of the same apparent form and size. Microsomes isolated from infected leaves contain MP. We show that the MP appears to behave as an integral ER membrane protein and is exposed on the cytosolic face of the ER. The importance of the association of MP with ER and its possible role in intracellular and intercellular spread of infection is discussed.