The yeast cell cortical protein Num1 integrates mitochondrial dynamics into cellular architecture
The yeast cell cortical protein Num1 integrates mitochondrial dynamics into cellular architecture
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DOI：
10.1242/jcs.126045
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发表时间：
2013-07-01
影响因子：
4
通讯作者：
Westermann, Benedikt
中科院分区：
文献类型：
--
作者：
Klecker, Till;Scholz, Dirk;Westermann, Benedikt
在细胞周期中，每个细胞器必须忠实地分配给子细胞。然而，控制细胞器遗传的机制仍然知之甚少。我们研究了细胞皮质蛋白 Num1 对酵母线粒体分配的贡献。活细胞显微镜显示，Num1 是线粒体附着到细胞皮层并保留在母细胞中所必需的。锚定位点的电子断层扫描显示质膜内陷直接接触线粒体外膜。嵌合质膜系链的表达挽救了 Delta num1 和 Delta mdm36 突变体的线粒体裂变缺陷。这些发现为遗传过程中线粒体动力学、固定化和保留的耦合提供了新的见解。
During the cell cycle each organelle has to be faithfully partitioned to the daughter cells. However, the mechanisms controlling organellar inheritance remain poorly understood. We studied the contribution of the cell cortex protein, Num1, to mitochondrial partitioning in yeast. Live-cell microscopy revealed that Num1 is required for attachment of mitochondria to the cell cortex and retention in mother cells. Electron tomography of anchoring sites revealed plasma membrane invaginations directly contacting the mitochondrial outer membrane. Expression of chimeric plasma membrane tethers rescued mitochondrial fission defects in Delta num1 and Delta mdm36 mutants. These findings provide new insights into the coupling of mitochondrial dynamics, immobilization, and retention during inheritance.