A Green Approach To Separate Spinach Pigments by Column Chromatography
A Green Approach To Separate Spinach Pigments by Column Chromatography
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DOI：
10.1021/ed300315z
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发表时间：
2013-06-01
影响因子：
3
通讯作者：
McDougal, Owen M.
中科院分区：
文献类型：
--
作者：
Johnston, Aubrey;Scaggs, Jon;McDougal, Owen M.
色谱法是一种用于化学分离的基本技术，其历史可以追溯到19世纪50年代。具体来说，柱色谱法，通常在介绍有机化学实验室教授，传统上涉及使用卤化或有害溶剂，新手学生经常过度使用。这种情况违背了绿色化学运动所强调的负责任的化学品和废物管理原则。自从这一运动开始以来，使用有害溶剂的传统分离方法已经得到了改进，以强调对更安全、危险性更低的材料的需求，并减少此类废物的产生。目前的实验强调可再生原料和回收利用的绿色化学原理，以尽量减少浪费，同时引入或加强常见的有机技术，包括溶剂萃取，柱色谱法，薄层色谱法分离和鉴定的光合色素从菠菜叶。学生将获得处理植物材料的实际经验，以分离和鉴定色素，β-胡萝卜素，叶黄素和叶绿素a，使用溶剂己烷和丙酮。该实验被设计为用作独立的单次实验室，或者，它可以与回收废丙酮的实验相结合，以进一步强调可持续的做法。
Chromatography has been a fundamental technique used for chemical separation that dates back to the 1850s. Specifically, column chromatography, typically taught in introductory organic chemistry laboratories, traditionally involves the use of halogenated or harmful solvents, which novice students often overuse. This situation runs contrary to the principles of responsible chemical and waste management emphasized by the green chemistry movement. Since this movement began, conventional means of separation using harmful solvents have been modified to emphasize the need for safer, less hazardous materials and the generation of such waste. The current experiment emphasizes the green chemical principles of renewable feedstocks and recycling to minimize waste, while simultaneously introducing or reinforcing common organic techniques, including solvent extraction, column chromatography, and thin-layer chromatography for the isolation and identification of photosynthetic pigments from spinach leaves. Students gain practical experience processing plant material to isolate and identify the pigments, beta-carotene, xanthophylls, and chlorophyll a, using the solvents hexane and acetone. This experiment was designed for use as a standalone single-session lab or, alternatively, it can be-coupled with an experiment to recycle waste acetone to further emphasize sustainable practices.