DNA migration and separation on surfaces with a microscale dielectrophoretic trap array.
DNA migration and separation on surfaces with a microscale dielectrophoretic trap array.
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使用微型介电泳陷阱阵列在表面上进行 DNA 迁移和分离。
DOI：
10.1103/physrevlett.98.088102
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发表时间：
2007
影响因子：
8.6
通讯作者：
Rafailovich,Miriam
中科院分区：
文献类型：
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作者：
Petersen,Eric;Li,Bingquan;Fang,Xiaohua;Luo,Haobin;Samuilov,Vladimir;Gersappe,Dilip;Sokolov,Jonathan;Chu,Benjamin;Rafailovich,Miriam
我们研究了在直流电场驱动下DNA链在局部介电泳陷阱中的表面迁移。通过调整的陷阱阵列的长度尺度，一个选定的带的DNA的分离完成与迁移率和碱基对的数量之间的比例指数类似于在毛细管电泳中获得的。然后，我们提供了一个模型，该模型预测的捕获和延伸的DNA链在介电泳陷阱负责的表面流动性和分离。
We studied the surface migration of DNA chains driven by a dc electric field across localized dielectrophoretic traps. By adjusting the length scale of the trap array, separation of a selected band of DNA was accomplished with a scaling exponent between mobility and number of base pairs similar to that obtained in capillary electrophoresis. We then provided a model, which predicts the trapping and extension of DNA chains at a dielectrophoretic trap responsible for the surface mobility and separation.