LONG-TERM INVITRO FUNCTION OF ADULT HEPATOCYTES IN A COLLAGEN SANDWICH CONFIGURATION
LONG-TERM INVITRO FUNCTION OF ADULT HEPATOCYTES IN A COLLAGEN SANDWICH CONFIGURATION
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DOI：
10.1021/bp00009a007
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发表时间：
1991-05-01
影响因子：
2.9
通讯作者：
YARMUSH, ML
中科院分区：
文献类型：
--
作者：
DUNN, JCY;TOMPKINS, RG;YARMUSH, ML
为了重建通常在肝脏中发现的细胞极性，将成年大鼠肝细胞夹在两层水合大鼠尾腱胶原基质之间。 在功能上，夹层肝细胞维持白蛋白，转铁蛋白，纤维蛋白原，胆汁酸和尿素的分泌至少6周，而在单层胶原凝胶上培养的细胞在1-2周内停止这种分泌。 在单层胶原凝胶上培养1周后，当应用第二层胶原凝胶时，肝细胞仍然可以恢复这些丧失的功能。 用于构建夹心系统的底物的确切性质似乎并不重要，只要它允许细胞附着即可。 在三明治系统中培养的肝细胞似乎保持类似于体内状态的肌动蛋白丝的分布，而在单层胶原凝胶上培养的细胞显示应力纤维的异常形成。 这些研究表明，细胞外元素的配置的简单操作可以显着改变培养的肝细胞的行为。
In an effort to reconstruct the cellular polarity normally found in the liver, adult rat hepatocytes were sandwiched between two layers of hydrated rat tail tendon collagen matrix. Functionally, sandwiched hepatocytes maintained the secretion of albumin, transferrin, fibrinogen, bile acids, and urea for at least 6 weeks, whereas cells cultured on a single layer of collagen gel ceased such secretion in 1-2 weeks. After 1 week of culture on a single layer of collagen gel, hepatocytes could still recover these lost functions when a second layer of collagen gel was applied. The exact nature of the substrate for constructing the sandwich system appeared to be unimportant as long as it allowed cellular attachment. Hepatocytes cultured in the sandwich system appeared to maintain a distribution of actin filaments similar to the in vivo state, whereas cells cultured on a single layer of collagen gel showed abnormal formation of stress fibers. These studies suggest that simple manipulations of the configuration of extracellular elements can dramatically alter the behavior of cultured hepatocytes.