High-Resolution Multi-Isotope Imaging Mass Spectrometry (MIMS) Imaging Applications in Stem Cell Biology.
High-Resolution Multi-Isotope Imaging Mass Spectrometry (MIMS) Imaging Applications in Stem Cell Biology.
复制标题
DOI：
10.1002/cpz1.290
复制
发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
Lechene CP
中科院分区：
文献类型：
--
作者：
McMahon G;Lechene CP
Multi-isotope imaging mass spectrometry (MIMS) allows the measurement of turnover of molecules within intracellular compartments with a spatial resolution down to 30 nm. We use molecules enriched in stable isotopes administered to animals by diet or injection, or to cells through the culture media. The stable isotopes used are in general 15N, 13C, 18O, and 2H. For stem cell studies we essentially use 15N-thymidine, 13C-thymidine and 81Br from BrdU. This protocol describes the practical use of MIMS with specific reference to applications in stem cell research. This includes choice and administration of stable isotope label(s), sample preparation, best practice for high resolution imaging secondary ion mass spectrometry using the Cameca NanoSIMS 50L and methods for robust statistical analysis of label incorporation in regions of interest (ROI).
